5F9G

pnGFP1.5-Y.Cro: circularly permuted green fluorescent protein (with a tyrosine-derived chromophore)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	pnGFP1.5-Y.Cro,Green fluorescent protein	polymer	259	28927.5	1	UniProt (P42212) Pfam (PF01353)	Aequorea victoria (Jellyfish)	circularly permuted green fluorescent protein
2	B (A)	water	water		18.0	16	Chemie (HOH)

Sequence modifications

A: 6 - 99 (UniProt: P42212)

PDB	External Database	Details
Met 1	-	initiating methionine
Gly 2	-	expression tag
Ser 3	-	expression tag
Ser 4	-	expression tag
Leu 5	-	expression tag
Lys 10	Asn 149	conflict
Thr 14	Met 153	conflict
Ala 24	Val 163	conflict
Gln 27	Lys 166	conflict
Val 32	Ile 171	conflict
Val 67	Ala 206	conflict
Leu 92	His 231	conflict
His 98	Tyr 237	conflict

A: 110 - 250 (UniProt: P42212)

PDB	External Database	Details
Val 100	-	linker
Asp 101	-	linker
Gly 102	-	linker
Gly 103	-	linker
Ser 104	-	linker
Gly 105	-	linker
Gly 106	-	linker
Thr 107	-	linker
Gly 108	-	linker
Val 109	-	linker
Arg 136	Ser 28	conflict
Arg 138	Ser 30	conflict
Asn 147	Tyr 39	conflict
Leu 172	Phe 64	conflict
Cro 173	Ser 65	chromophore
Cro 173	Tyr 66	chromophore
Cro 173	Gly 67	chromophore
Thr 211	Asn 105	conflict
Ser 251	-	expression tag
His 252	-	expression tag
His 253	-	expression tag
His 254	-	expression tag
His 255	-	expression tag
His 256	-	expression tag
His 257	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	28927.5
All*	Total formula weight	28927.5

*Water molecules are not included.