5F9G

pnGFP1.5-Y.Cro: circularly permuted green fluorescent protein (with a tyrosine-derived chromophore)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-29
Detector	ADSC QUANTUM 315
Wavelength(s)	0.987
Spacegroup name	P 43 21 2
Unit cell lengths	57.258, 57.258, 185.392
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.682 - 2.772
R-factor	0.2075
Rwork	0.203
R-free	0.29470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1emb
RMSD bond length	0.010
RMSD bond angle	1.442
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.800
High resolution limit [Å]	2.750	2.750
Rmerge	0.089	0.920
Number of reflections	14821
<I/σ(I)>	80.4	8
Completeness [%]	99.8	100
Redundancy	24.7	27.1
CC(1/2)	0.968

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	pnGFP1.5-Y.Cro in buffer 50mM Hepes pH 7.5, 0.3M NaCl, 1mM beta-mercaptoethanol, A280=52; Crystals were grown in drops of 1ul protein solution: 1ul well solution containing 20-22% PEG 3350, 0.25M potassium thiocyanate, 0.1M Tris pH 8.0