5F85

Crystal structure of Drosophila Poglut1 (Rumi) complexed with its substrate protein (EGF repeat) and UDP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	O-glucosyltransferase rumi	polymer	402	46820.2	1	UniProt (Q8T045) Pfam (PF05686)	Drosophila melanogaster (Fruit fly)
2	B (B)	Coagulation factor IX	polymer	50	5710.2	1	UniProt (P00740) Pfam (PF00008)	Homo sapiens (Human)	Christmas factor,Plasma thromboplastin component,PTC
3	C (A)	URIDINE-5'-DIPHOSPHATE	non-polymer		404.2	1	Chemie (UDP)
4	D (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
5	E, F, G, H, I (A)	SULFATE ION	non-polymer		96.1	5	Chemie (SO4)
6	J, K (A, B)	water	water		18.0	125	Chemie (HOH)

Sequence modifications

A: 21 - 407 (UniProt: Q8T045)

PDB	External Database	Details
Ala 13	-	expression tag
Ala 14	-	expression tag
Gln 15	-	expression tag
Pro 16	-	expression tag
Ala 17	-	expression tag
Glu 18	-	expression tag
Ala 19	-	expression tag
Leu 20	-	expression tag
Ala 408	-	expression tag
Arg 409	-	expression tag
Ala 410	-	expression tag
Leu 411	-	expression tag
Val 412	-	expression tag
Pro 413	-	expression tag
Arg 414	-	expression tag

B: 46 - 84 (UniProt: P00740)

PDB	External Database	Details
Met 43	-	initiating methionine
Asp 44	-	expression tag
Ile 45	-	expression tag
Leu 85	-	expression tag
Glu 86	-	expression tag
His 87	-	expression tag
His 88	-	expression tag
His 89	-	expression tag
His 90	-	expression tag
His 91	-	expression tag
His 92	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	52530.4
Non-Polymers*	Number of molecules	7
	Total formula weight	976.6
All*	Total formula weight	53507.0

*Water molecules are not included.