5F6C

The structure of E. coli RNase E catalytically inactive mutant with RNA bound

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Ribonuclease E	polymer	512	57790.8	1	UniProt (P21513) Pfam (PF00575) Pfam (PF10150) Pfam (PF20833)	Escherichia coli (strain K12)	RNase E
2	B (B)	Ribonuclease E	polymer	513	57861.9	1	UniProt (P21513) Pfam (PF00575) Pfam (PF10150) Pfam (PF20833)	Escherichia coli (strain K12)	RNase E
3	C (C)	RNA (5'-R(P*GP*U)-3')	polymer	2	606.4	1		Escherichia coli
4	D (E)	RNA (5'-R(P*GP*UP*G)-3')	polymer	3	951.6	1		Escherichia coli
5	E, G (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	F (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
7	H (A)	water	water		18.0	1	Chemie (HOH)

Sequence modifications

A: 1 - 510 (UniProt: P21513)

PDB	External Database	Details
Arg -1	-	expression tag
His 0	-	expression tag
Arg 303	Asp 303	engineered mutation
Arg 346	Asp 346	engineered mutation

B: 1 - 511 (UniProt: P21513)

PDB	External Database	Details
Arg -1	-	expression tag
His 0	-	expression tag
Arg 303	Asp 303	engineered mutation
Arg 346	Asp 346	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	117210.7
Non-Polymers*	Number of molecules	3
	Total formula weight	114.0
All*	Total formula weight	117324.7

*Water molecules are not included.