5F6C
The structure of E. coli RNase E catalytically inactive mutant with RNA bound
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Ribonuclease E | polymer | 512 | 57790.8 | 1 | UniProt (P21513) Pfam (PF00575) Pfam (PF10150) Pfam (PF20833) In PDB | Escherichia coli (strain K12) | RNase E |
2 | B | Ribonuclease E | polymer | 513 | 57861.9 | 1 | UniProt (P21513) Pfam (PF00575) Pfam (PF10150) Pfam (PF20833) In PDB | Escherichia coli (strain K12) | RNase E |
3 | C | RNA (5'-R(P*GP*U)-3') | polymer | 2 | 606.4 | 1 | Escherichia coli | ||
4 | E | RNA (5'-R(P*GP*UP*G)-3') | polymer | 3 | 951.6 | 1 | Escherichia coli | ||
5 | A, B | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
6 | A | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
7 | water | water | 18.0 | 1 | Chemie (HOH) |
Sequence modifications
A: 1 - 510 (UniProt: P21513)
B: 1 - 511 (UniProt: P21513)
PDB | External Database | Details |
---|---|---|
Arg -1 | - | expression tag |
His 0 | - | expression tag |
Arg 303 | Asp 303 | engineered mutation |
Arg 346 | Asp 346 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Arg -1 | - | expression tag |
His 0 | - | expression tag |
Arg 303 | Asp 303 | engineered mutation |
Arg 346 | Asp 346 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 117210.7 | |
Non-Polymers* | Number of molecules | 3 |
Total formula weight | 114.0 | |
All* | Total formula weight | 117324.7 |