5F6C

The structure of E. coli RNase E catalytically inactive mutant with RNA bound

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003676	molecular_function	nucleic acid binding
A	0003723	molecular_function	RNA binding
A	0004540	molecular_function	RNA nuclease activity
A	0006396	biological_process	RNA processing
A	0008995	molecular_function	ribonuclease E activity
B	0003676	molecular_function	nucleic acid binding
B	0003723	molecular_function	RNA binding
B	0004540	molecular_function	RNA nuclease activity
B	0006396	biological_process	RNA processing
B	0008995	molecular_function	ribonuclease E activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	binding site for residue MG A 601

Chain	Residue
A	ASP26
A	LEU27
A	ASP28
A	ARG335
A	ASP338

---

site_id	AC2
Number of Residues	4
Details	binding site for residue ZN A 602

Chain	Residue
A	CYS404
A	CYS407
B	CYS404
B	CYS407

---

site_id	AC3
Number of Residues	3
Details	binding site for residue MG B 601

Chain	Residue
B	ASP28
B	ARG216
B	SER393

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	160
Details	Domain: {"description":"S1 motif","evidences":[{"source":"HAMAP-Rule","id":"MF_00970","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI2
Number of Residues	110
Details	Region: {"description":"Interaction with RNA"}

Chain

Residue

Details

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site_id	SWS_FT_FI3
Number of Residues	2
Details	Region: {"description":"Interaction with RNA 5'-terminal monophosphate"}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	6
Details	Region: {"description":"Required for zinc-mediated homotetramerization and catalytic activity"}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"16237448","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"15779893","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"18682225","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---