5F0L

Structure of retromer VPS26-VPS35 subunits bound to SNX3 and DMT1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Vacuolar protein sorting-associated protein 35	polymer	462	53288.3	1	UniProt (Q96QK1) Pfam (PF03635) In PDB	Homo sapiens (Human)	hVPS35,Maternal-embryonic 3,Vesicle protein sorting 35
2	B	Vacuolar protein sorting-associated protein 26A	polymer	317	37164.8	1	UniProt (O75436) Pfam (PF03643) In PDB	Homo sapiens (Human)	Vesicle protein sorting 26A,hVPS26
3	C	Sorting nexin-3	polymer	167	19193.8	1	UniProt (O60493) Pfam (PF00787) In PDB	Homo sapiens (Human)	Protein SDP3
4	D	Natural resistance-associated macrophage protein 2	polymer	24	2660.0	1	UniProt (P49281) In PDB	Homo sapiens (Human)	NRAMP 2,Divalent cation transporter 1,Divalent metal transporter 1,DMT-1,Solute carrier family 11 member 2
5	A, C, B	SULFATE ION	non-polymer		96.1	10	Chemie (SO4)
6	A, C, B	GLYCEROL	non-polymer		92.1	6	Chemie (GOL)
7	B, A, C	1,2-ETHANEDIOL	non-polymer		62.1	18	Chemie (EDO)

Sequence modifications

A: 14 - 470 (UniProt: Q96QK1)

PDB	External Database	Details
Gly 9	-	expression tag
Ala 10	-	expression tag
Met 11	-	expression tag
Gly 12	-	expression tag
Ser 13	-	expression tag

C: 1 - 162 (UniProt: O60493)

PDB	External Database	Details
Gly -4	-	expression tag
Ala -3	-	expression tag
Met -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	112306.9
Non-Polymers*	Number of molecules	34
	Total formula weight	2630.4
All*	Total formula weight	114937.3

*Water molecules are not included.