5F0L
Structure of retromer VPS26-VPS35 subunits bound to SNX3 and DMT1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97903 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 371.370, 75.210, 57.450 |
Unit cell angles | 90.00, 98.56, 90.00 |
Refinement procedure
Resolution | 56.800 - 3.200 |
R-factor | 0.2141 |
Rwork | 0.212 |
R-free | 0.25880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5f0j |
RMSD bond length | 0.007 |
RMSD bond angle | 1.153 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 56.820 | 3.380 | |
High resolution limit [Å] | 3.190 | 9.450 | 3.190 |
Rmerge | 0.183 | 0.040 | 0.929 |
Rmeas | 0.198 | 0.044 | 1.006 |
Total number of observations | 178697 | ||
Number of reflections | 26241 | 1059 | 4074 |
<I/σ(I)> | 9.3 | 37.64 | 1.82 |
Completeness [%] | 99.3 | 99.3 | 96.2 |
Redundancy | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 0.75 M AmSO4, 0.1 M MES pH 6.0, 15% Glycerol |