5F0J

Structure of retromer VPS26-VPS35 subunits bound to SNX3

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Vacuolar protein sorting-associated protein 35	polymer	462	53288.3	1	UniProt (Q96QK1) Pfam (PF03635)	Homo sapiens (Human)	hVPS35,Maternal-embryonic 3,Vesicle protein sorting 35
2	B (B)	Vacuolar protein sorting-associated protein 26A	polymer	341	40239.6	1	UniProt (O75436) Pfam (PF03643)	Homo sapiens (Human)	Vesicle protein sorting 26A,hVPS26
3	C (C)	Sorting nexin-3	polymer	167	19193.8	1	UniProt (O60493) Pfam (PF00787)	Homo sapiens (Human)	Protein SDP3
4	D, E, FA, GA, HA... (A, C, B)	SULFATE ION	non-polymer		96.1	6	Chemie (SO4)
5	F, IA, Q, R, S (A, C, B)	GLYCEROL	non-polymer		92.1	5	Chemie (GOL)
6	AA, BA, CA, DA, EA... (B, C, A)	1,2-ETHANEDIOL	non-polymer		62.1	24	Chemie (EDO)
7	MA, NA, OA (A, B, C)	water	water		18.0	95	Chemie (HOH)

Sequence modifications

A: 14 - 470 (UniProt: Q96QK1)

PDB	External Database	Details
Gly 9	-	expression tag
Ala 10	-	expression tag
Met 11	-	expression tag
Gly 12	-	expression tag
Ser 13	-	expression tag

B: 2 - 326 (UniProt: O75436)

PDB	External Database	Details
Mse -1	-	expression tag
Gly 0	-	expression tag
Mse 1	-	expression tag
Mse 327	-	expression tag
Gly 328	-	expression tag
Leu 329	-	expression tag
Val 330	-	expression tag
Pro 331	-	expression tag
Arg 332	-	expression tag
Gly 333	-	expression tag
Ser 334	-	expression tag
His 335	-	expression tag
His 336	-	expression tag
His 337	-	expression tag
His 338	-	expression tag
His 339	-	expression tag

C: 1 - 162 (UniProt: O60493)

PDB	External Database	Details
Gly -4	-	expression tag
Ala -3	-	expression tag
Met -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	112721.7
Non-Polymers*	Number of molecules	35
	Total formula weight	2526.5
All*	Total formula weight	115248.2

*Water molecules are not included.