5F09
Structure of inactive GCPII mutant in complex with beta-citryl glutamate
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Glutamate carboxypeptidase 2 | polymer | 739 | 83142.5 | 1 | UniProt (Q04609) Pfam (PF02225) Pfam (PF04389) Pfam (PF04253) In PDB | Homo sapiens (Human) | Cell growth-inhibiting gene 27 protein,Folate hydrolase 1,Folylpoly-gamma-glutamate carboxypeptidase,FGCP,Glutamate carboxypeptidase II,GCPII,Membrane glutamate carboxypeptidase,mGCP,N-acetylated-alpha-linked acidic dipeptidase I,NAALADase I,Prostate-specific membrane antigen,PSMA,Pteroylpoly-gamma-glutamate carboxypeptidase |
2 | B, C | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 2 | In PDB GlyTouCan (G42666HT) | |||
3 | D | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 1 | In PDB GlyTouCan (G15407YE) | |||
4 | E | alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 748.7 | 1 | In PDB GlyTouCan (G81315DD) | |||
5 | A | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 3 | Chemie (NAG) | |||
6 | A | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
7 | A | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
8 | A | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
9 | A | beta-citryl-L-glutamic acid | non-polymer | 321.2 | 1 | Chemie (BC8) | |||
10 | A | TRIETHYLENE GLYCOL | non-polymer | 150.2 | 1 | Chemie (PGE) | |||
11 | water | water | 18.0 | 561 | Chemie (HOH) |
Sequence modifications
A: 44 - 750 (UniProt: Q04609)
PDB | External Database | Details |
---|---|---|
Arg 12 | - | expression tag |
Ser 13 | - | expression tag |
Gly 14 | - | expression tag |
Leu 15 | - | expression tag |
Asn 16 | - | expression tag |
Asp 17 | - | expression tag |
Ile 18 | - | expression tag |
Phe 19 | - | expression tag |
Glu 20 | - | expression tag |
Ala 21 | - | expression tag |
Gln 22 | - | expression tag |
Lys 23 | - | expression tag |
Ile 24 | - | expression tag |
Glu 25 | - | expression tag |
Trp 26 | - | expression tag |
His 27 | - | expression tag |
Glu 28 | - | expression tag |
Gly 29 | - | expression tag |
Ser 30 | - | expression tag |
Gly 31 | - | expression tag |
Ser 32 | - | expression tag |
Gly 33 | - | expression tag |
Ser 34 | - | expression tag |
Glu 35 | - | expression tag |
Asn 36 | - | expression tag |
Leu 37 | - | expression tag |
Tyr 38 | - | expression tag |
Phe 39 | - | expression tag |
Gln 40 | - | expression tag |
Gly 41 | - | expression tag |
Arg 42 | - | expression tag |
Ser 43 | - | expression tag |
Ala 424 | Glu 424 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 83142.5 | |
Branched | Number of molecules | 4 |
Total formula weight | 2184.0 | |
Non-Polymers* | Number of molecules | 9 |
Total formula weight | 1341.4 | |
All* | Total formula weight | 86667.9 |