5EZV

X-ray crystal structure of AMP-activated protein kinase alpha-2/alpha-1 RIM chimaera (alpha-2(1-347)/alpha-1(349-401)/alpha-2(397-end) beta-1 gamma-1) co-crystallized with C2 (5-(5-hydroxyl-isoxazol-3-yl)-furan-2-phosphonic acid)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	5'-AMP-activated protein kinase catalytic subunit alpha-2/alpha-1 RIM SWAP chimera	polymer	560	63902.7	2	UniProt (P54646) UniProt (Q13131) Pfam (PF00069) Pfam (PF21147) Pfam (PF16579) In PDB	Homo sapiens (Human)	AMPK subunit alpha-2,Acetyl-CoA carboxylase kinase,ACACA kinase,Hydroxymethylglutaryl-CoA reductase kinase,HMGCR kinase,AMPK subunit alpha-1,Acetyl-CoA carboxylase kinase,ACACA kinase,Hydroxymethylglutaryl-CoA reductase kinase,HMGCR kinase,Tau-protein kinase PRKAA1
2	B, D	5'-AMP-activated protein kinase subunit beta-1	polymer	270	30504.3	2	UniProt (Q9Y478) Pfam (PF16561) Pfam (PF04739) In PDB	Homo sapiens (Human)	AMPKb
3	E, F	5'-AMP-activated protein kinase subunit gamma-1	polymer	336	38225.9	2	UniProt (P54619) Pfam (PF00571) In PDB	Homo sapiens (Human)	AMPKg
4	A, C	STAUROSPORINE	non-polymer		466.5	2	Chemie (STU)
5	A, C	3-[4-(2-hydroxyphenyl)phenyl]-4-oxidanyl-6-oxidanylidene-7H-thieno[2,3-b]pyridine-5-carbonitrile	non-polymer		360.4	2	Chemie (C1V)
6	E, F	5-(5-hydroxyl-isoxazol-3-yl)-furan-2-phosphonic acid	non-polymer		231.1	4	Chemie (C2Z)
7		water	water		18.0	84	Chemie (HOH)

Sequence modifications

A, C: 2 - 347 (UniProt: P54646)

PDB	External Database	Details
Met -13	-	initiating methionine
Gly -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Ser -2	-	expression tag
Gln -1	-	expression tag
Asp 0	-	expression tag
Pro 1	-	expression tag

A, C: 348 - 390 (UniProt: Q13131)
A, C: 391 - 546 (UniProt: P54646)
E, F: 2 - 331 (UniProt: P54619)

PDB	External Database	Details
Met -4	-	initiating methionine
Ala -3	-	expression tag
Asp -2	-	expression tag
Leu -1	-	expression tag
Asn 0	-	expression tag
Trp 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	265265.8
Non-Polymers*	Number of molecules	8
	Total formula weight	2578.2
All*	Total formula weight	267844.1

*Water molecules are not included.