5EZV
X-ray crystal structure of AMP-activated protein kinase alpha-2/alpha-1 RIM chimaera (alpha-2(1-347)/alpha-1(349-401)/alpha-2(397-end) beta-1 gamma-1) co-crystallized with C2 (5-(5-hydroxyl-isoxazol-3-yl)-furan-2-phosphonic acid)
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004672 | molecular_function | protein kinase activity |
| A | 0004679 | molecular_function | AMP-activated protein kinase activity |
| A | 0005524 | molecular_function | ATP binding |
| A | 0006468 | biological_process | protein phosphorylation |
| B | 0005515 | molecular_function | protein binding |
| B | 0005634 | cellular_component | nucleus |
| B | 0005654 | cellular_component | nucleoplasm |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0006633 | biological_process | fatty acid biosynthetic process |
| B | 0007165 | biological_process | signal transduction |
| B | 0019901 | molecular_function | protein kinase binding |
| B | 0031588 | cellular_component | nucleotide-activated protein kinase complex |
| B | 0031669 | biological_process | cellular response to nutrient levels |
| B | 0032991 | cellular_component | protein-containing complex |
| B | 0035878 | biological_process | nail development |
| B | 0120162 | biological_process | positive regulation of cold-induced thermogenesis |
| C | 0004672 | molecular_function | protein kinase activity |
| C | 0004679 | molecular_function | AMP-activated protein kinase activity |
| C | 0005524 | molecular_function | ATP binding |
| C | 0006468 | biological_process | protein phosphorylation |
| D | 0005515 | molecular_function | protein binding |
| D | 0005634 | cellular_component | nucleus |
| D | 0005654 | cellular_component | nucleoplasm |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005829 | cellular_component | cytosol |
| D | 0006633 | biological_process | fatty acid biosynthetic process |
| D | 0007165 | biological_process | signal transduction |
| D | 0019901 | molecular_function | protein kinase binding |
| D | 0031588 | cellular_component | nucleotide-activated protein kinase complex |
| D | 0031669 | biological_process | cellular response to nutrient levels |
| D | 0032991 | cellular_component | protein-containing complex |
| D | 0035878 | biological_process | nail development |
| D | 0120162 | biological_process | positive regulation of cold-induced thermogenesis |
| E | 0004691 | molecular_function | cAMP-dependent protein kinase activity |
| E | 0005515 | molecular_function | protein binding |
| E | 0005524 | molecular_function | ATP binding |
| E | 0005634 | cellular_component | nucleus |
| E | 0005654 | cellular_component | nucleoplasm |
| E | 0005737 | cellular_component | cytoplasm |
| E | 0005829 | cellular_component | cytosol |
| E | 0006110 | biological_process | regulation of glycolytic process |
| E | 0006468 | biological_process | protein phosphorylation |
| E | 0006633 | biological_process | fatty acid biosynthetic process |
| E | 0007165 | biological_process | signal transduction |
| E | 0007283 | biological_process | spermatogenesis |
| E | 0008603 | molecular_function | cAMP-dependent protein kinase regulator activity |
| E | 0016020 | cellular_component | membrane |
| E | 0016208 | molecular_function | AMP binding |
| E | 0019887 | molecular_function | protein kinase regulator activity |
| E | 0019901 | molecular_function | protein kinase binding |
| E | 0031588 | cellular_component | nucleotide-activated protein kinase complex |
| E | 0031669 | biological_process | cellular response to nutrient levels |
| E | 0042149 | biological_process | cellular response to glucose starvation |
| E | 0043531 | molecular_function | ADP binding |
| E | 0043609 | biological_process | regulation of carbon utilization |
| E | 0045722 | biological_process | positive regulation of gluconeogenesis |
| E | 0045860 | biological_process | positive regulation of protein kinase activity |
| E | 0051170 | biological_process | import into nucleus |
| F | 0004691 | molecular_function | cAMP-dependent protein kinase activity |
| F | 0005515 | molecular_function | protein binding |
| F | 0005524 | molecular_function | ATP binding |
| F | 0005634 | cellular_component | nucleus |
| F | 0005654 | cellular_component | nucleoplasm |
| F | 0005737 | cellular_component | cytoplasm |
| F | 0005829 | cellular_component | cytosol |
| F | 0006110 | biological_process | regulation of glycolytic process |
| F | 0006468 | biological_process | protein phosphorylation |
| F | 0006633 | biological_process | fatty acid biosynthetic process |
| F | 0007165 | biological_process | signal transduction |
| F | 0007283 | biological_process | spermatogenesis |
| F | 0008603 | molecular_function | cAMP-dependent protein kinase regulator activity |
| F | 0016020 | cellular_component | membrane |
| F | 0016208 | molecular_function | AMP binding |
| F | 0019887 | molecular_function | protein kinase regulator activity |
| F | 0019901 | molecular_function | protein kinase binding |
| F | 0031588 | cellular_component | nucleotide-activated protein kinase complex |
| F | 0031669 | biological_process | cellular response to nutrient levels |
| F | 0042149 | biological_process | cellular response to glucose starvation |
| F | 0043531 | molecular_function | ADP binding |
| F | 0043609 | biological_process | regulation of carbon utilization |
| F | 0045722 | biological_process | positive regulation of gluconeogenesis |
| F | 0045860 | biological_process | positive regulation of protein kinase activity |
| F | 0051170 | biological_process | import into nucleus |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 14 |
| Details | binding site for residue STU A 601 |
| Chain | Residue |
| A | LEU22 |
| A | VAL96 |
| A | GLU100 |
| A | GLU143 |
| A | ASN144 |
| A | LEU146 |
| A | GLY23 |
| A | VAL24 |
| A | GLY25 |
| A | VAL30 |
| A | ALA43 |
| A | MET93 |
| A | GLU94 |
| A | TYR95 |
| site_id | AC2 |
| Number of Residues | 10 |
| Details | binding site for residue C1V A 602 |
| Chain | Residue |
| A | LEU18 |
| A | GLY19 |
| A | LYS29 |
| A | LYS31 |
| A | ILE46 |
| A | ASP88 |
| B | ARG83 |
| B | THR106 |
| B | SEP108 |
| B | VAL113 |
| site_id | AC3 |
| Number of Residues | 14 |
| Details | binding site for residue STU C 601 |
| Chain | Residue |
| C | LEU22 |
| C | GLY23 |
| C | VAL24 |
| C | GLY25 |
| C | VAL30 |
| C | ALA43 |
| C | GLU94 |
| C | TYR95 |
| C | VAL96 |
| C | GLU100 |
| C | GLU143 |
| C | ASN144 |
| C | LEU146 |
| C | ASP157 |
| site_id | AC4 |
| Number of Residues | 12 |
| Details | binding site for residue C1V C 602 |
| Chain | Residue |
| C | VAL11 |
| C | LEU18 |
| C | GLY19 |
| C | LYS29 |
| C | LYS31 |
| C | ILE46 |
| C | ASN48 |
| C | ASP88 |
| D | ARG83 |
| D | ARG107 |
| D | SEP108 |
| D | VAL113 |
| site_id | AC5 |
| Number of Residues | 8 |
| Details | binding site for residue C2Z E 401 |
| Chain | Residue |
| E | HIS151 |
| E | ARG152 |
| E | SER226 |
| E | HIS298 |
| E | ARG299 |
| E | SER314 |
| E | SER316 |
| E | C2Z402 |
| site_id | AC6 |
| Number of Residues | 10 |
| Details | binding site for residue C2Z E 402 |
| Chain | Residue |
| E | ARG70 |
| E | THR87 |
| E | THR89 |
| E | HIS151 |
| E | SER226 |
| E | SER242 |
| E | LYS243 |
| E | PHE244 |
| E | ARG299 |
| E | C2Z401 |
| site_id | AC7 |
| Number of Residues | 8 |
| Details | binding site for residue C2Z F 401 |
| Chain | Residue |
| F | HIS151 |
| F | ARG152 |
| F | SER226 |
| F | HIS298 |
| F | ARG299 |
| F | SER314 |
| F | SER316 |
| F | C2Z402 |
| site_id | AC8 |
| Number of Residues | 11 |
| Details | binding site for residue C2Z F 402 |
| Chain | Residue |
| F | ARG70 |
| F | THR87 |
| F | THR89 |
| F | HIS151 |
| F | SER226 |
| F | SER242 |
| F | LYS243 |
| F | PHE244 |
| F | HIS298 |
| F | ARG299 |
| F | C2Z401 |
Functional Information from PROSITE/UniProt
| site_id | PS00107 |
| Number of Residues | 24 |
| Details | PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGVGTFGKVKiGehqltghk..........VAVK |
| Chain | Residue | Details |
| A | LEU22-LYS45 |
| site_id | PS00108 |
| Number of Residues | 13 |
| Details | PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. VvHrDLKpeNVLL |
| Chain | Residue | Details |
| A | VAL135-LEU147 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 16 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:P80385 |
| Chain | Residue | Details |
| E | ARG70 | |
| F | MET85 | |
| F | VAL130 | |
| F | ARG152 | |
| F | LYS170 | |
| F | SER242 | |
| F | ARG269 | |
| F | LEU277 | |
| E | MET85 | |
| E | VAL130 | |
| E | ARG152 | |
| E | LYS170 | |
| E | SER242 | |
| E | ARG269 | |
| E | LEU277 | |
| F | ARG70 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 12 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:17452784, ECO:0000269|PubMed:24352254, ECO:0000269|PubMed:25412657, ECO:0007744|PDB:4CFF |
| Chain | Residue | Details |
| E | HIS151 | |
| F | SER226 | |
| F | HIS298 | |
| F | SER314 | |
| E | THR200 | |
| E | ALA205 | |
| E | SER226 | |
| E | HIS298 | |
| E | SER314 | |
| F | HIS151 | |
| F | THR200 | |
| F | ALA205 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphoserine; by ULK1 => ECO:0000250|UniProtKB:P80385 |
| Chain | Residue | Details |
| E | SER261 | |
| E | SER270 | |
| F | SER261 | |
| F | SER270 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphothreonine; by ULK1 => ECO:0000250|UniProtKB:P80385 |
| Chain | Residue | Details |
| E | THR263 | |
| F | THR263 | |
| D | SER24 | |
| D | SER25 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:18691976, ECO:0007744|PubMed:19369195, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:23186163 |
| Chain | Residue | Details |
| B | SER40 | |
| D | SER40 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569 |
| Chain | Residue | Details |
| B | SER96 | |
| D | SER96 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P80386 |
| Chain | Residue | Details |
| B | SER101 | |
| D | SER101 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:18088087, ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:18691976, ECO:0007744|PubMed:19369195, ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569 |
| Chain | Residue | Details |
| B | SEP108 | |
| D | SEP108 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphothreonine => ECO:0007744|PubMed:19369195 |
| Chain | Residue | Details |
| B | THR148 | |
| D | THR148 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:Q9R078 |
| Chain | Residue | Details |
| B | SER182 | |
| D | SER182 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 2 |
| Details | LIPID: N-myristoyl glycine => ECO:0000269|PubMed:21680840 |
| Chain | Residue | Details |
| B | GLY2 | |
| D | GLY2 |
