5EW5

Crystal Structure of Colicin E9 In Complex with Its Immunity Protein Im9

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Colicin-E9	polymer	582	61563.1	4	UniProt (P09883) Pfam (PF03515) Pfam (PF11570) Pfam (PF21431) In PDB	Escherichia coli
2	E, F, G, H	Colicin-E9 immunity protein	polymer	94	10663.7	4	UniProt (P13479) Pfam (PF01320) In PDB	Escherichia coli	ImmE9,Microcin-E9 immunity protein
3		water	water		18.0	22	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 582 (UniProt: P09883)

PDB	External Database	Details
Cys 324	Tyr 324	engineered mutation
Cys 447	Leu 447	engineered mutation
Ala 448	Asp 448	engineered mutation
Met 449	Lys 449	engineered mutation

E, F, G, H: 1 - 86 (UniProt: P13479)

PDB	External Database	Details
Leu 87	-	expression tag
Glu 88	-	expression tag
His 89	-	expression tag
His 90	-	expression tag
His 91	-	expression tag
His 92	-	expression tag
His 93	-	expression tag
His 94	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	288907.2
All*	Total formula weight	288907.2

*Water molecules are not included.