5EW5

Crystal Structure of Colicin E9 In Complex with Its Immunity Protein Im9

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004518	molecular_function	nuclease activity
A	0004519	molecular_function	endonuclease activity
A	0005515	molecular_function	protein binding
A	0005727	cellular_component	extrachromosomal circular DNA
A	0016787	molecular_function	hydrolase activity
A	0019904	molecular_function	protein domain specific binding
A	0031640	biological_process	killing of cells of another organism
A	0032991	cellular_component	protein-containing complex
A	0042742	biological_process	defense response to bacterium
A	0046872	molecular_function	metal ion binding
B	0004518	molecular_function	nuclease activity
B	0004519	molecular_function	endonuclease activity
B	0005515	molecular_function	protein binding
B	0005727	cellular_component	extrachromosomal circular DNA
B	0016787	molecular_function	hydrolase activity
B	0019904	molecular_function	protein domain specific binding
B	0031640	biological_process	killing of cells of another organism
B	0032991	cellular_component	protein-containing complex
B	0042742	biological_process	defense response to bacterium
B	0046872	molecular_function	metal ion binding
C	0004518	molecular_function	nuclease activity
C	0004519	molecular_function	endonuclease activity
C	0005515	molecular_function	protein binding
C	0005727	cellular_component	extrachromosomal circular DNA
C	0016787	molecular_function	hydrolase activity
C	0019904	molecular_function	protein domain specific binding
C	0031640	biological_process	killing of cells of another organism
C	0032991	cellular_component	protein-containing complex
C	0042742	biological_process	defense response to bacterium
C	0046872	molecular_function	metal ion binding
D	0004518	molecular_function	nuclease activity
D	0004519	molecular_function	endonuclease activity
D	0005515	molecular_function	protein binding
D	0005727	cellular_component	extrachromosomal circular DNA
D	0016787	molecular_function	hydrolase activity
D	0019904	molecular_function	protein domain specific binding
D	0031640	biological_process	killing of cells of another organism
D	0032991	cellular_component	protein-containing complex
D	0042742	biological_process	defense response to bacterium
D	0046872	molecular_function	metal ion binding
E	0005515	molecular_function	protein binding
E	0015643	molecular_function	toxic substance binding
E	0019904	molecular_function	protein domain specific binding
E	0030153	biological_process	bacteriocin immunity
E	0032991	cellular_component	protein-containing complex
F	0005515	molecular_function	protein binding
F	0015643	molecular_function	toxic substance binding
F	0019904	molecular_function	protein domain specific binding
F	0030153	biological_process	bacteriocin immunity
F	0032991	cellular_component	protein-containing complex
G	0005515	molecular_function	protein binding
G	0015643	molecular_function	toxic substance binding
G	0019904	molecular_function	protein domain specific binding
G	0030153	biological_process	bacteriocin immunity
G	0032991	cellular_component	protein-containing complex
H	0005515	molecular_function	protein binding
H	0015643	molecular_function	toxic substance binding
H	0019904	molecular_function	protein domain specific binding
H	0030153	biological_process	bacteriocin immunity
H	0032991	cellular_component	protein-containing complex

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	600
Details	Region: {"description":"Disordered","evidences":[{"source":"SAM","id":"MobiDB-lite","evidenceCode":"ECO:0000256"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	232
Details	Compositional bias: {"description":"Basic and acidic residues","evidences":[{"source":"SAM","id":"MobiDB-lite","evidenceCode":"ECO:0000256"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	52
Details	Compositional bias: {"description":"Low complexity","evidences":[{"source":"SAM","id":"MobiDB-lite","evidenceCode":"ECO:0000256"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	12
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	7
Details	M-CSA 791

Chain	Residue	Details
A	ARG453	electrostatic stabiliser
A	ARG544	electrostatic stabiliser
A	GLU548	electrostatic stabiliser
A	HIS550	metal ligand
A	HIS551	increase nucleophilicity, proton acceptor, proton donor
A	HIS575	metal ligand
A	HIS579	metal ligand

---

site_id	MCSA2
Number of Residues	7
Details	M-CSA 791

Chain	Residue	Details
B	ARG453	electrostatic stabiliser
B	ARG544	electrostatic stabiliser
B	GLU548	electrostatic stabiliser
B	HIS550	metal ligand
B	HIS551	increase nucleophilicity, proton acceptor, proton donor
B	HIS575	metal ligand
B	HIS579	metal ligand

---

site_id	MCSA3
Number of Residues	7
Details	M-CSA 791

Chain	Residue	Details
C	ARG453	electrostatic stabiliser
C	ARG544	electrostatic stabiliser
C	GLU548	electrostatic stabiliser
C	HIS550	metal ligand
C	HIS551	increase nucleophilicity, proton acceptor, proton donor
C	HIS575	metal ligand
C	HIS579	metal ligand

---

site_id	MCSA4
Number of Residues	7
Details	M-CSA 791

Chain	Residue	Details
D	ARG453	electrostatic stabiliser
D	ARG544	electrostatic stabiliser
D	GLU548	electrostatic stabiliser
D	HIS550	metal ligand
D	HIS551	increase nucleophilicity, proton acceptor, proton donor
D	HIS575	metal ligand
D	HIS579	metal ligand

---