5ELN

Crystal Structure of Lysyl-tRNA Synthetase from Cryptosporidium parvum complexed with L-lysine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Lysine--tRNA ligase	polymer	535	61459.1	4	UniProt (Q5CR27) Pfam (PF01336) Pfam (PF00152) In PDB	Cryptosporidium parvum (strain Iowa II)	Lysyl-tRNA synthetase
2	A, B, C, D	LYSINE	non-polymer		147.2	4	Chemie (LYS)
3	A, B, C, D	1,2-ETHANEDIOL	non-polymer		62.1	9	Chemie (EDO)
4	C	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
5		water	water		18.0	1942	Chemie (HOH)

Sequence modifications

A, B, C, D: 46 - 559 (UniProt: Q5CR27)

PDB	External Database	Details
Met 25	-	initiating methionine
Ala 26	-	expression tag
His 27	-	expression tag
His 28	-	expression tag
His 29	-	expression tag
His 30	-	expression tag
His 31	-	expression tag
His 32	-	expression tag
Met 33	-	expression tag
Gly 34	-	expression tag
Thr 35	-	expression tag
Leu 36	-	expression tag
Glu 37	-	expression tag
Ala 38	-	expression tag
Gln 39	-	expression tag
Thr 40	-	expression tag
Gln 41	-	expression tag
Gly 42	-	expression tag
Pro 43	-	expression tag
Gly 44	-	expression tag
Ser 45	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	245836.4
Non-Polymers*	Number of molecules	14
	Total formula weight	1239.5
All*	Total formula weight	247075.9

*Water molecules are not included.