5EKE

Structure of the polyisoprenyl-phosphate glycosyltransferase GtrB (F215A mutant)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Uncharacterized glycosyltransferase sll0501	polymer	341	39243.3	4	UniProt (Q55487) Pfam (PF00535)	Synechocystis sp. (strain PCC 6803 / Kazusa)
2	E, G, I, K (A, B, C, D)	URIDINE-5'-DIPHOSPHATE	non-polymer		404.2	4	Chemie (UDP) PubChem (1158) PubChem (6031) PubChem (25323044) PubChem (25323048) PubChem (44298965) PubChem (53486216) PubChem (60098857) PubChem (57448939) PubChem (3084486) PubChem (91746160) PubChem (6101632) PubChem (92332560) PubChem (92332561) PubChem (92332562) PubChem (92332563) PubChem (93088131) PubChem (93630083) PubChem (6604175) PubChem (11811538) PubChem (12049241)
3	F, H, J, L (A, B, C, D)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG) PubChem (888)

Sequence modifications

A, B, C, D: 2 - 318 (UniProt: Q55487)

PDB	External Database	Details
Met -22	-	initiating methionine
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
Ser -15	-	expression tag
Ser -14	-	expression tag
Gly -13	-	expression tag
Val -12	-	expression tag
Asp -11	-	expression tag
Leu -10	-	expression tag
Gly -9	-	expression tag
Thr -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Ser -1	-	expression tag
Asn 0	-	expression tag
Ala 1	-	expression tag
Ala 215	Phe 215	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	156973.3
Non-Polymers*	Number of molecules	8
	Total formula weight	1713.9
All*	Total formula weight	158687.2

*Water molecules are not included.