5EKE

Structure of the polyisoprenyl-phosphate glycosyltransferase GtrB (F215A mutant)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Uncharacterized glycosyltransferase sll0501	polymer	341	39243.3	4	UniProt (Q55487) Pfam (PF00535)	Synechocystis sp. (strain PCC 6803 / Kazusa)
2	E, G, I, K (A, B, C, D)	URIDINE-5'-DIPHOSPHATE	non-polymer		404.2	4	Chemie (UDP)
3	F, H, J, L (A, B, C, D)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)

Sequence modifications

A, B, C, D: 2 - 318 (UniProt: Q55487)

PDB	External Database	Details
Met -22	-	initiating methionine
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
Ser -15	-	expression tag
Ser -14	-	expression tag
Gly -13	-	expression tag
Val -12	-	expression tag
Asp -11	-	expression tag
Leu -10	-	expression tag
Gly -9	-	expression tag
Thr -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Ser -1	-	expression tag
Asn 0	-	expression tag
Ala 1	-	expression tag
Ala 215	Phe 215	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	156973.3
Non-Polymers*	Number of molecules	8
	Total formula weight	1713.9
All*	Total formula weight	158687.2

*Water molecules are not included.