5EE7

Crystal structure of the human glucagon receptor (GCGR) in complex with the antagonist MK-0893

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Glucagon receptor,Endolysin,Glucagon receptor	polymer	452	51279.5	1	UniProt (P47871) UniProt (P00720) Pfam (PF00002) Pfam (PF00959) In PDB	Homo sapiens (Human)	GL-R,Lysis protein,Lysozyme,Muramidase,GL-R
2	A	3-[[4-[(1~{S})-1-[3-[3,5-bis(chloranyl)phenyl]-5-(6-methoxynaphthalen-2-yl)pyrazol-1-yl]ethyl]phenyl]carbonylamino]propanoic acid	non-polymer		588.5	1	Chemie (5MV)
3	A	OLEIC ACID	non-polymer		282.5	14	Chemie (OLA)
4	A	3,6,9,12,15,18,21,24-OCTAOXAHEXACOSAN-1-OL	non-polymer		398.5	1	Chemie (PE5)
5	A	L(+)-TARTARIC ACID	non-polymer		150.1	1	Chemie (TLA)
6		water	water		18.0	24	Chemie (HOH)

Sequence modifications

A: 136 - 254 (UniProt: P47871)

PDB	External Database	Details
Gly 137	Met 137	engineered mutation
Ala 154	Gly 154	engineered mutation
Ala 173	Arg 173	engineered mutation
Leu 182	Ala 182	engineered mutation
Ala 190	Ser 190	engineered mutation
Phe 193	Val 193	engineered mutation
Glu 207	Gly 207	engineered mutation
Ala 223	Gly 223	engineered mutation

A: 255 - 1158 (UniProt: P00720)

PDB	External Database	Details
Leu 255	Met 1	linker
Gly 1010	Arg 12	engineered mutation
Thr 1052	Cys 54	engineered mutation
Ala 1095	Cys 97	engineered mutation
Arg 1135	Ile 137	engineered mutation
Tyr 1159	-	linker

A: 259 - 417 (UniProt: P47871)

PDB	External Database	Details
Ala 276	Met 276	engineered mutation
Ala 344	Lys 344	engineered mutation
Phe 362	Glu 362	engineered mutation
Ala 387	Phe 387	engineered mutation
Ala 418	-	expression tag
Ala 419	-	expression tag
Ala 420	-	expression tag
His 421	-	expression tag
His 422	-	expression tag
His 423	-	expression tag
His 424	-	expression tag
His 425	-	expression tag
His 426	-	expression tag
His 427	-	expression tag
His 428	-	expression tag
His 429	-	expression tag
His 430	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	51279.5
Non-Polymers*	Number of molecules	17
	Total formula weight	5091.5
All*	Total formula weight	56371.0

*Water molecules are not included.