5EE7

Crystal structure of the human glucagon receptor (GCGR) in complex with the antagonist MK-0893

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	37.583, 71.476, 183.108
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.971 - 2.500
R-factor	0.2275
Rwork	0.226
R-free	0.26250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4l6r
RMSD bond length	0.004
RMSD bond angle	0.796
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.730	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.157	0.768
Number of reflections	16065
<I/σ(I)>	6	1.6
Completeness [%]	91.1	90.2
Redundancy	3.3	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	6	293.15	ADA BUFFER, SODIUM POTASSIUM TARTRATE, PEG 400