5EE7
Crystal structure of the human glucagon receptor (GCGR) in complex with the antagonist MK-0893
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.583, 71.476, 183.108 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.971 - 2.500 |
| R-factor | 0.2275 |
| Rwork | 0.226 |
| R-free | 0.26250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4l6r |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.796 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.730 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.157 | 0.768 |
| Number of reflections | 16065 | |
| <I/σ(I)> | 6 | 1.6 |
| Completeness [%] | 91.1 | 90.2 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 6 | 293.15 | ADA BUFFER, SODIUM POTASSIUM TARTRATE, PEG 400 |
