5EE5

Structure of human ARL1 in complex with the DCB domain of BIG1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Brefeldin A-inhibited guanine nucleotide-exchange protein 1	polymer	213	24173.0	1	UniProt (Q9Y6D6) Pfam (PF16213)	Homo sapiens (Human)	Brefeldin A-inhibited GEP 1,ADP-ribosylation factor guanine nucleotide-exchange factor 1,p200 ARF guanine nucleotide exchange factor,p200 ARF-GEP1
2	B (B)	ADP-ribosylation factor-like protein 1	polymer	171	19371.3	1	UniProt (P40616) Pfam (PF00025)	Homo sapiens (Human)
3	AA, BA, C, D, E... (B, A)	SODIUM ION	non-polymer		23.0	14	Chemie (NA)
4	CA, I (B, A)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5	DA, J, K, L, M... (B, A)	CHLORIDE ION	non-polymer		35.5	7	Chemie (CL)
6	P (B)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP)
7	Q, R, S, T (B)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
8	EA (B)	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
9	FA, GA (A, B)	water	water		18.0	41	Chemie (HOH)

Sequence modifications

A: 1 - 229 (UniProt: Q9Y6D6)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
-	Ser 52	deletion
-	Pro 53	deletion
-	Pro 54	deletion
-	His 55	deletion
-	Gly 56	deletion
-	Glu 57	deletion
-	Ala 58	deletion
-	Lys 59	deletion
-	Ala 60	deletion
-	Gly 61	deletion
-	Ser 62	deletion
-	Ser 63	deletion
-	Thr 64	deletion
-	Leu 65	deletion
-	Pro 66	deletion
-	Pro 67	deletion
-	Val 68	deletion
-	Lys 69	deletion
-	Ser 70	deletion

B: 15 - 181 (UniProt: P40616)

PDB	External Database	Details
Gly 11	-	expression tag
Ser 12	-	expression tag
His 13	-	expression tag
Met 14	-	expression tag
Leu 71	Gln 71	conflict

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	43544.3
Non-Polymers*	Number of molecules	29
	Total formula weight	1433.7
All*	Total formula weight	44977.9

*Water molecules are not included.