5E9G

Structural insights of isocitrate lyases from Magnaporthe oryzae

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Isocitrate lyase	polymer	560	62625.8	4	UniProt (P0CT06) Pfam (PF00463)	Magnaporthe oryzae 70-15 (Rice blast fungus)	ICL,ISOCITRASE,Isocitratase,Methylisocitrate lyase,MICA,Threo-D(S)-isocitrate glyoxylate-lyase
2	E, H, K, M (A, B, C, D)	GLYOXYLIC ACID	non-polymer		74.0	4	Chemie (GLV)
3	F, I, N (A, B, D)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
4	G, J, L, O (A, B, C, D)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
5	P, Q, R, S (A, B, C, D)	water	water		18.0	304	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 547 (UniProt: P0CT06)

PDB	External Database	Details
Gly 219	Ala 219	engineered mutation
Ala 548	-	expression tag
Ala 549	-	expression tag
Ala 550	-	expression tag
Leu 551	-	expression tag
Glu 552	-	expression tag
His 553	-	expression tag
His 554	-	expression tag
His 555	-	expression tag
His 556	-	expression tag
His 557	-	expression tag
His 558	-	expression tag
His 559	-	expression tag
His 560	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	250503.2
Non-Polymers*	Number of molecules	11
	Total formula weight	669.6
All*	Total formula weight	251172.8

*Water molecules are not included.