5E8M

Crystal structure of human heparanase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Heparanase	polymer	389	43733.3	1	UniProt (Q9Y251) Pfam (PF03662) In PDB	Homo sapiens (Human)	Endo-glucoronidase,Heparanase-1,Hpa1, glycoside hydrolase
2	B	Heparanase	polymer	77	8542.8	1	UniProt (Q9Y251) In PDB	Homo sapiens (Human)	Endo-glucoronidase,Heparanase-1,Hpa1, glycoside hydrolase
3	C	alpha-L-fucopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		367.3	1	In PDB GlyTouCan (G86851RC)
4	A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)
5	A, B	1,2-ETHANEDIOL	non-polymer		62.1	15	Chemie (EDO)
6	A	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
7		water	water		18.0	249	Chemie (HOH)

Sequence modifications

A: 158 - 543 (UniProt: Q9Y251)

PDB	External Database	Details
Asp 155	-	expression tag
Pro 156	-	expression tag
Gly 157	-	expression tag
Arg 307	Lys 307	variant

B: 1 - 74 (UniProt: Q9Y251)

PDB	External Database	Details
Asp -2	-	expression tag
Pro -1	-	expression tag
Gly 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	52276.1
Branched	Number of molecules	1
	Total formula weight	367.3
Non-Polymers*	Number of molecules	22
	Total formula weight	1922.2
All*	Total formula weight	54565.7

*Water molecules are not included.