5E1Q
Mutant (D415G) GH97 alpha-galactosidase in complex with Gal-Lac
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Retaining alpha-galactosidase | polymer | 655 | 74347.1 | 2 | UniProt (Q8A6L0) Pfam (PF14508) Pfam (PF10566) Pfam (PF14509) | Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482) | BtGH97b,Melibiase |
| 2 | C, D (C, D) | alpha-D-galactopyranose-(1-1)-[beta-D-galactopyranose-(1-4)]beta-D-glucopyranose | branched | 504.4 | 2 | In PDB | |||
| 3 | E, G (A, B) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 4 | F, H, I (A, B) | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
| 5 | J, K (A, B) | water | water | 18.0 | 763 | Chemie (HOH) |
Sequence modifications
A, B: 27 - 662 (UniProt: Q8A6L0)
| PDB | External Database | Details |
|---|---|---|
| Met 8 | - | expression tag |
| Asn 9 | - | expression tag |
| His 10 | - | expression tag |
| Lys 11 | - | expression tag |
| Val 12 | - | expression tag |
| His 13 | - | expression tag |
| His 14 | - | expression tag |
| His 15 | - | expression tag |
| His 16 | - | expression tag |
| His 17 | - | expression tag |
| His 18 | - | expression tag |
| Ile 19 | - | expression tag |
| Glu 20 | - | expression tag |
| Gly 21 | - | expression tag |
| Arg 22 | - | expression tag |
| His 23 | - | expression tag |
| Met 24 | - | expression tag |
| Glu 25 | - | expression tag |
| Leu 26 | - | expression tag |
| Gly 415 | Asp 415 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 148694.2 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 1008.9 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 356.4 | |
| All* | Total formula weight | 150059.5 |
