5DXE

Estrogen Receptor Alpha Ligand Binding Domain Y537S Mutant in Complex with Stapled Peptide SRC2-P4 and Estradiol

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Estrogen receptor	polymer	261	29912.4	2	UniProt (P03372) Pfam (PF00104)	Homo sapiens (Human)	ER,ER-alpha,Estradiol receptor,Nuclear receptor subfamily 3 group A member 1
2	C, D (C, D)	Nuclear receptor coactivator 2	polymer	13	1415.7	2	UniProt (Q15596)	Homo sapiens	NCoA-2,Class E basic helix-loop-helix protein 75,bHLHe75,Transcriptional intermediary factor 2,hTIF2
3	E, F (A, B)	ESTRADIOL	non-polymer		272.4	2	Chemie (EST)
4	G, H, I, J (A, B, C, D)	water	water		18.0	398	Chemie (HOH)

Sequence modifications

A, B: 297 - 554 (UniProt: P03372)

PDB	External Database	Details
Met 294	-	initiating methionine
Asp 295	-	expression tag
Pro 296	-	expression tag
Ser 537	Tyr 537	engineered mutation

C, D: 2 - 12 (UniProt: Q15596)

PDB	External Database	Details
Ace 1	-	expression tag
Mk8 4	Ile 689	conflict
Nh2 13	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	62656.2
Non-Polymers*	Number of molecules	2
	Total formula weight	544.8
All*	Total formula weight	63201.0

*Water molecules are not included.