5DWZ

Structural and functional characterization of PqsBC, a condensing enzyme in the biosynthesis of the Pseudomonas aeruginosa quinolone signal

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G (C, D, F, H)	3-oxoacyl-[acyl-carrier-protein] synthase 3	polymer	350	38327.5	4	UniProt (A0A0C6EZ24) Pfam (PF08545) Pfam (PF08541) UniProt (by SIFTS) (Q9I4X1)	Pseudomonas aeruginosa	Beta-keto-ACP synthase,Beta-keto-acyl-acyl-carrier-like protein synthase,PQS biosynthesis protein PqsC,Uncharacterized protein
2	B, D, F, H (B, A, E, G)	3-oxoacyl-(Acyl carrier protein) synthase III	polymer	283	30531.8	4	UniProt (A0A072ZZD2) UniProt (by SIFTS) (Q9I4X2)	Pseudomonas aeruginosa	Beta-keto-ACP synthase,Beta-keto-acyl-acyl-carrier-like protein synthase,PQS biosynthesis protein PqsB,PqsB
3	FA, GA, HA, I, IA... (A, F, C, E, H...)	PHOSPHATE ION	non-polymer		95.0	20	Chemie (PO4)
4	AA, L, M, R, S... (D, C, B)	GLYCEROL	non-polymer		92.1	7	Chemie (GOL)
5	BA, N, O, TA (D, C, H)	3[N-MORPHOLINO]PROPANE SULFONIC ACID	non-polymer		209.3	4	Chemie (MPO)
6	P (C)	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	1	Chemie (TRS)
7	EA, Q (D, C)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
8	V (B)	TRIETHYLENE GLYCOL	non-polymer		150.2	1	Chemie (PGE)
9	CA, DA, UA (D, H)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	3	Chemie (PEG)
10	NA (E)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
11	AB, BB, CB, VA, WA... (E, H, G, C, B...)	water	water		18.0	1095	Chemie (HOH)

Sequence modifications

C, D, F, H: 2 - 348 (UniProt: A0A0C6EZ24)

PDB	External Database	Details
Ser -1	-	expression tag
Ala 0	-	expression tag
Gly 1	-	expression tag
Ala 129	Cys 129	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	275437.2
Non-Polymers*	Number of molecules	39
	Total formula weight	4065.7
All*	Total formula weight	279502.9

*Water molecules are not included.