5DTZ

Crystal structure of rsFolder in the fluorescent on-state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Green fluorescent protein	polymer	262	29374.1	4	UniProt (P42212) Pfam (PF01353) In PDB	Aequorea victoria (Jellyfish)
2	C, D	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	3	Chemie (PEG)
3		water	water		18.0	1004	Chemie (HOH)

Sequence modifications

A, B, C, D: 3 - 239 (UniProt: P42212)

PDB	External Database	Details
Met -22	-	initiating methionine
Gly -21	-	expression tag
Ser -20	-	expression tag
Ser -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Leu -9	-	expression tag
Val -8	-	expression tag
Pro -7	-	expression tag
Arg -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
His -3	-	expression tag
Met -2	-	expression tag
Ala -1	-	expression tag
Thr 0	-	expression tag
Met 1	-	expression tag
Val 2	-	expression tag
Arg 31	Ser 30	engineered mutation
Asn 40	Tyr 39	conflict
Leu 65	Phe 64	conflict
Pia 68	Ser 65	chromophore
Pia 68	Tyr 66	chromophore
Pia 68	Gly 67	chromophore
Leu 70	Gln 69	engineered mutation
Arg 81	Gln 80	engineered mutation
Ser 100	Phe 99	conflict
Thr 106	Asn 105	conflict
Phe 146	Tyr 145	conflict
Thr 154	Met 153	conflict
Ser 164	Val 163	engineered mutation
Val 172	Ile 171	conflict
Lys 207	Ala 206	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	117496.5
Non-Polymers*	Number of molecules	3
	Total formula weight	318.4
All*	Total formula weight	117814.8

*Water molecules are not included.