5DTZ
Crystal structure of rsFolder in the fluorescent on-state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 142.470, 134.560, 51.720 |
| Unit cell angles | 90.00, 106.05, 90.00 |
Refinement procedure
| Resolution | 95.970 - 1.500 |
| R-factor | 0.17518 |
| Rwork | 0.174 |
| R-free | 0.20160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b3p |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.396 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.970 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 144849 | |
| <I/σ(I)> | 9 | 1.1 |
| Completeness [%] | 97.1 | 81.62 |
| Redundancy | 4 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1 M Tris, 20% PEG3350 |
