5DSG
Structure of the M4 muscarinic acetylcholine receptor (M4-mT4L) bound to tiotropium
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Muscarinic acetylcholine receptor M4,Endolysin,Endolysin,Muscarinic acetylcholine receptor M4 | polymer | 422 | 47800.7 | 2 | UniProt (P08173) UniProt (P00720) Pfam (PF00001) Pfam (PF00959) | Homo sapiens (Human) | Lysis protein,Lysozyme,Muramidase,Lysis protein,Lysozyme,Muramidase |
| 2 | C, L (A, B) | (1R,2R,4S,5S,7S)-7-{[hydroxy(dithiophen-2-yl)acetyl]oxy}-9,9-dimethyl-3-oxa-9-azoniatricyclo[3.3.1.0~2,4~]nonane | non-polymer | 392.5 | 2 | Chemie (0HK) | |||
| 3 | D, E, K, N, O (A, B) | OLEIC ACID | non-polymer | 282.5 | 5 | Chemie (OLA) | |||
| 4 | F (A) | {[-(BIS-CARBOXYMETHYL-AMINO)-ETHYL]-CARBOXYMETHYL-AMINO}-ACETIC ACID | non-polymer | 292.2 | 1 | Chemie (EDT) | |||
| 5 | G, P (A, B) | HEXAETHYLENE GLYCOL | non-polymer | 282.3 | 2 | Chemie (P6G) | |||
| 6 | H, I, J, M, Q (A, B) | (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate | non-polymer | 356.5 | 5 | Chemie (OLC) | |||
| 7 | R (B) | 1-(2-METHOXY-ETHOXY)-2-{2-[2-(2-METHOXY-ETHOXY]-ETHOXY}-ETHANE | non-polymer | 266.3 | 1 | Chemie (PG6) | |||
| 8 | S, T (A, B) | water | water | 18.0 | 27 | Chemie (HOH) |
Sequence modifications
A, B: 22 - 226 (UniProt: P08173)
A, B: 1002 - 1011 (UniProt: P00720)
A, B: 1018 - 1118 (UniProt: P00720)
A, B: 390 - 478 (UniProt: P08173)
| PDB | External Database | Details |
|---|---|---|
| Gly 20 | - | expression tag |
| Pro 21 | - | expression tag |
A, B: 1018 - 1118 (UniProt: P00720)
| PDB | External Database | Details |
|---|---|---|
| Gly 1012 | - | linker |
| Gly 1013 | - | linker |
| Gly 1014 | - | linker |
| Ser 1015 | - | linker |
| Gly 1016 | - | linker |
| Gly 1017 | - | linker |
| Ala 1054 | Cys 97 | engineered mutation |
| Arg 1094 | Ile 137 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Arg 479 | - | expression tag |
| His 480 | - | expression tag |
| His 481 | - | expression tag |
| His 482 | - | expression tag |
| His 483 | - | expression tag |
| His 484 | - | expression tag |
| His 485 | - | expression tag |
| His 486 | - | expression tag |
| His 487 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 95601.3 | |
| Non-Polymers* | Number of molecules | 16 |
| Total formula weight | 5103.3 | |
| All* | Total formula weight | 100704.6 |
