5DSG
Structure of the M4 muscarinic acetylcholine receptor (M4-mT4L) bound to tiotropium
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Muscarinic acetylcholine receptor M4,Endolysin,Endolysin,Muscarinic acetylcholine receptor M4 | polymer | 422 | 47800.7 | 2 | UniProt (P08173) UniProt (P00720) Pfam (PF00001) Pfam (PF00959) | Homo sapiens (Human) | Lysis protein,Lysozyme,Muramidase,Lysis protein,Lysozyme,Muramidase |
| 2 | C, L (A, B) | (1R,2R,4S,5S,7S)-7-{[hydroxy(dithiophen-2-yl)acetyl]oxy}-9,9-dimethyl-3-oxa-9-azoniatricyclo[3.3.1.0~2,4~]nonane | non-polymer | 392.5 | 2 | Chemie (0HK) PubChem (50909376) PubChem (58667495) PubChem (59084585) PubChem (59168855) PubChem (59288009) PubChem (60003260) PubChem (656611) PubChem (87909275) PubChem (3086655) PubChem (60831) PubChem (131951) PubChem (123134303) PubChem (5487427) PubChem (5748851) PubChem (98047845) PubChem (9804844) PubChem (122130800) PubChem (11598033) PubChem (124527228) PubChem (15057856) | |||
| 3 | D, E, K, N, O (A, B) | OLEIC ACID | non-polymer | 282.5 | 5 | Chemie (OLA) PubChem (965) PubChem (445639) PubChem (637517) | |||
| 4 | F (A) | {[-(BIS-CARBOXYMETHYL-AMINO)-ETHYL]-CARBOXYMETHYL-AMINO}-ACETIC ACID | non-polymer | 292.2 | 1 | Chemie (EDT) PubChem (6049) | |||
| 5 | G, P (A, B) | HEXAETHYLENE GLYCOL | non-polymer | 282.3 | 2 | Chemie (P6G) PubChem (17472) | |||
| 6 | H, I, J, M, Q (A, B) | (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate | non-polymer | 356.5 | 5 | Chemie (OLC) PubChem (25021708) PubChem (46840179) PubChem (54294271) PubChem (54294270) PubChem (33022) PubChem (5283468) PubChem (5364833) PubChem (11451146) PubChem (12178130) | |||
| 7 | R (B) | 1-(2-METHOXY-ETHOXY)-2-{2-[2-(2-METHOXY-ETHOXY]-ETHOXY}-ETHANE | non-polymer | 266.3 | 1 | Chemie (PG6) PubChem (70931) | |||
| 8 | S, T (A, B) | water | water | 18.0 | 27 | Chemie (HOH) |
Sequence modifications
A, B: 22 - 226 (UniProt: P08173)
A, B: 1002 - 1011 (UniProt: P00720)
A, B: 1018 - 1118 (UniProt: P00720)
A, B: 390 - 478 (UniProt: P08173)
| PDB | External Database | Details |
|---|---|---|
| Gly 20 | - | expression tag |
| Pro 21 | - | expression tag |
A, B: 1018 - 1118 (UniProt: P00720)
| PDB | External Database | Details |
|---|---|---|
| Gly 1012 | - | linker |
| Gly 1013 | - | linker |
| Gly 1014 | - | linker |
| Ser 1015 | - | linker |
| Gly 1016 | - | linker |
| Gly 1017 | - | linker |
| Ala 1054 | Cys 97 | engineered mutation |
| Arg 1094 | Ile 137 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Arg 479 | - | expression tag |
| His 480 | - | expression tag |
| His 481 | - | expression tag |
| His 482 | - | expression tag |
| His 483 | - | expression tag |
| His 484 | - | expression tag |
| His 485 | - | expression tag |
| His 486 | - | expression tag |
| His 487 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 95601.3 | |
| Non-Polymers* | Number of molecules | 16 |
| Total formula weight | 5103.3 | |
| All* | Total formula weight | 100704.6 |
