5DS5
Crystal structure the Escherichia coli Cas1-Cas2 complex bound to protospacer DNA and Mg
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | CRISPR-associated endonuclease Cas1 | polymer | 306 | 33322.5 | 4 | UniProt (Q46896) Pfam (PF01867) | Escherichia coli (strain K12) | |
| 2 | E, F (E, F) | CRISPR-associated endoribonuclease Cas2 | polymer | 104 | 11684.5 | 2 | UniProt (P45956) Pfam (PF09707) | Escherichia coli (strain K12) | |
| 3 | G (G) | DNA (28-MER) | polymer | 28 | 8680.6 | 1 | Enterobacteria phage M13 | ||
| 4 | H (H) | DNA (28-MER) | polymer | 28 | 8534.5 | 1 | Enterobacteria phage M13 | ||
| 5 | I, J, K, L, M (B, D, G, H) | MAGNESIUM ION | non-polymer | 24.3 | 5 | Chemie (MG) |
Sequence modifications
A, B, C, D: 1 - 305 (UniProt: Q46896)
E, F: 1 - 94 (UniProt: P45956)
| PDB | External Database | Details |
|---|---|---|
| Ser 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met 0 | - | initiating methionine |
| Gly 95 | - | expression tag |
| Ser 96 | - | expression tag |
| Ser 97 | - | expression tag |
| Glu 98 | - | expression tag |
| Asn 99 | - | expression tag |
| Leu 100 | - | expression tag |
| Tyr 101 | - | expression tag |
| Phe 102 | - | expression tag |
| Gln 103 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 173874.0 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 121.5 | |
| All* | Total formula weight | 173995.6 |
