5DS5
Crystal structure the Escherichia coli Cas1-Cas2 complex bound to protospacer DNA and Mg
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2015-07-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.11587 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.656, 165.928, 167.258 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.475 - 2.951 |
R-factor | 0.2348 |
Rwork | 0.234 |
R-free | 0.25640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4p6i |
RMSD bond length | 0.003 |
RMSD bond angle | 0.718 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.475 | 3.060 |
High resolution limit [Å] | 2.950 | 2.950 |
Rmerge | 0.108 | 0.863 |
Number of reflections | 44960 | |
<I/σ(I)> | 9.8 | 1.4 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 7.9 | 8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 50 mM MES, pH 6.1, 10% isopropanol and 20 mM MgCl2 |