5DJ8

Fc Heterodimer Design 7.7 D399M/Y407A + T366V/K409I

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Ig gamma-1 chain C region	polymer	227	25537.0	1	UniProt (P01857) Pfam (PF07654)	Homo sapiens (Human)
2	B (B)	Ig gamma-1 chain C region	polymer	240	26964.3	1	UniProt (P01857) Pfam (PF07654)	Homo sapiens (Human)
3	C (C)	Fc-III peptide	polymer	13	1533.7	1		synthetic construct
4	D, E (D, E)	beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1422.3	2	In PDB GlyTouCan (G61334IA)
5	F, G, H (A, B, C)	water	water		18.0	104	Chemie (HOH)

Sequence modifications

A: 221 - 447 (UniProt: P01857)

PDB	External Database	Details
Glu 356	Asp 239	variant
Met 358	Leu 241	variant
Met 399	Asp 282	engineered mutation
Ala 407	Tyr 290	engineered mutation

B: 221 - 447 (UniProt: P01857)

PDB	External Database	Details
His 208	-	expression tag
His 209	-	expression tag
His 210	-	expression tag
His 211	-	expression tag
His 212	-	expression tag
His 213	-	expression tag
His 214	-	expression tag
His 215	-	expression tag
Ser 216	-	expression tag
Gly 217	-	expression tag
Ser 218	-	expression tag
Gly 219	-	expression tag
Ser 220	-	expression tag
Glu 252	Met 135	engineered mutation
Ala 253	Ile 136	engineered mutation
Glu 356	Asp 239	variant
Met 358	Leu 241	variant
Val 366	Thr 249	engineered mutation
Ile 409	Lys 292	engineered mutation
Ala 435	His 318	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	54035.1
Branched	Number of molecules	2
	Total formula weight	2844.6
All*	Total formula weight	56879.7

*Water molecules are not included.