5DJ8
Fc Heterodimer Design 7.7 D399M/Y407A + T366V/K409I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 193 |
Detector technology | CCD |
Collection date | 2013-10-02 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.97931 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.508, 67.669, 78.376 |
Unit cell angles | 90.00, 104.34, 90.00 |
Refinement procedure
Resolution | 28.800 - 2.400 |
R-factor | 0.21674 |
Rwork | 0.214 |
R-free | 0.26025 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.518 |
Data scaling software | SCALA (3.3.20) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0017) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 28.800 | 67.669 | 2.530 |
High resolution limit [Å] | 2.400 | 7.590 | 2.400 |
Rmerge | 0.047 | 0.455 | |
Rmeas | 0.121 | ||
Rpim | 0.064 | 0.030 | 0.315 |
Total number of observations | 82649 | 2636 | 10554 |
Number of reflections | 23694 | ||
<I/σ(I)> | 7.3 | 14.2 | 2.4 |
Completeness [%] | 98.0 | 94.2 | 96.4 |
Redundancy | 3.5 | 3.5 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 294 | 31% PEG 3350 + 100mM Ammonium Iodide |