5DIS
Crystal structure of a CRM1-RanGTP-SPN1 export complex bound to a 113 amino acid FG-repeat containing fragment of Nup214
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Exportin-1 | polymer | 1044 | 120395.7 | 1 | UniProt (O14980) Pfam (PF03810) Pfam (PF08389) Pfam (PF18777) Pfam (PF18784) Pfam (PF18787) Pfam (PF08767) | Homo sapiens (Human) | Exp1,Chromosome region maintenance 1 protein homolog |
| 2 | B (B) | GTP-binding nuclear protein Ran | polymer | 172 | 19812.1 | 1 | UniProt (P62826) Pfam (PF00071) | Homo sapiens (Human) | Androgen receptor-associated protein 24,GTPase Ran,Ras-like protein TC4,Ras-related nuclear protein |
| 3 | C (C) | Snurportin-1 | polymer | 289 | 33276.5 | 1 | UniProt (O95149) Pfam (PF11538) Pfam (PF21974) | Homo sapiens (Human) | RNA U transporter 1 |
| 4 | D (D) | Maltose-binding periplasmic protein,Nuclear pore complex protein Nup214 | polymer | 479 | 50577.5 | 1 | UniProt (P0AEX9) UniProt (P35658) Pfam (PF01547) Pfam (PF18617) | Escherichia coli (strain K12) | MBP,MMBP,Maltodextrin-binding protein,214 kDa nucleoporin,Nucleoporin Nup214,Protein CAN |
| 5 | E (E) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 1 | In PDB BIRD (PRD_900001) GlyTouCan (G07411ON) | alpha-maltose | ||
| 6 | F, I (A, C) | PROLINE | non-polymer | 115.1 | 2 | Chemie (PRO) PubChem (614) PubChem (8988) PubChem (145742) | |||
| 7 | G (B) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466) | |||
| 8 | H (B) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) PubChem (888) | |||
| 9 | J, K (A, B) | water | water | 18.0 | 12 | Chemie (HOH) |
Sequence modifications
B: 8 - 179 (UniProt: P62826)
C: 1 - 287 (UniProt: O95149)
D: 6 - 361 (UniProt: P0AEX9)
D: 1916 - 2027 (UniProt: P35658)
| PDB | External Database | Details |
|---|---|---|
| Leu 69 | Gln 69 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | linker |
| Ser 0 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Ala 362 | - | linker |
| Ala 363 | - | linker |
| Ala 364 | - | linker |
| Gln 365 | - | linker |
| Thr 366 | - | linker |
| Asn 367 | - | linker |
| Ala 368 | - | linker |
| Ala 1912 | - | linker |
| Ala 1913 | - | linker |
| Glu 1914 | - | linker |
| Phe 1915 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 224061.8 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 342.3 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 777.7 | |
| All* | Total formula weight | 225181.9 |
