5DIS
Crystal structure of a CRM1-RanGTP-SPN1 export complex bound to a 113 amino acid FG-repeat containing fragment of Nup214
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Exportin-1 | polymer | 1044 | 120395.7 | 1 | UniProt (O14980) Pfam (PF03810) Pfam (PF08389) Pfam (PF18777) Pfam (PF18784) Pfam (PF18787) Pfam (PF08767) In PDB | Homo sapiens (Human) | Exp1,Chromosome region maintenance 1 protein homolog |
| 2 | B | GTP-binding nuclear protein Ran | polymer | 172 | 19812.1 | 1 | UniProt (P62826) Pfam (PF00071) In PDB | Homo sapiens (Human) | Androgen receptor-associated protein 24,GTPase Ran,Ras-like protein TC4,Ras-related nuclear protein |
| 3 | C | Snurportin-1 | polymer | 289 | 33276.5 | 1 | UniProt (O95149) Pfam (PF11538) In PDB | Homo sapiens (Human) | RNA U transporter 1 |
| 4 | D | Maltose-binding periplasmic protein,Nuclear pore complex protein Nup214 | polymer | 479 | 50577.5 | 1 | UniProt (P0AEX9) UniProt (P35658) Pfam (PF18617) Pfam (PF01547) In PDB | Escherichia coli (strain K12) | MBP,MMBP,Maltodextrin-binding protein,214 kDa nucleoporin,Nucleoporin Nup214,Protein CAN |
| 5 | E | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 1 | In PDB BIRD (PRD_900001) GlyTouCan (G07411ON) | alpha-maltose | ||
| 6 | A, C | PROLINE | non-polymer | 115.1 | 2 | Chemie (PRO) | |||
| 7 | B | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) | |||
| 8 | B | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 9 | water | water | 18.0 | 12 | Chemie (HOH) |
Sequence modifications
B: 8 - 179 (UniProt: P62826)
C: 1 - 287 (UniProt: O95149)
D: 6 - 361 (UniProt: P0AEX9)
D: 1916 - 2027 (UniProt: P35658)
| PDB | External Database | Details |
|---|---|---|
| Leu 69 | Gln 69 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | linker |
| Ser 0 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Ala 362 | - | linker |
| Ala 363 | - | linker |
| Ala 364 | - | linker |
| Gln 365 | - | linker |
| Thr 366 | - | linker |
| Asn 367 | - | linker |
| Ala 368 | - | linker |
| Ala 1912 | - | linker |
| Ala 1913 | - | linker |
| Glu 1914 | - | linker |
| Phe 1915 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 224061.8 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 342.3 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 777.7 | |
| All* | Total formula weight | 225181.9 |
