5DFE

70S termination complex containing E. coli RF2

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	QV, XV	P-site tRNA fMet	polymer	77	24802.8	2	GenBank (1016582139)	Escherichia coli
2	QX, XX	messenger RNA	polymer	25	8196.0	2		synthetic construct
3	QY, XY	Peptide chain release factor 2	polymer	380	42991.4	2	UniProt (P07012) Pfam (PF03462) Pfam (PF00472) In PDB	Escherichia coli (strain K12)	RF-2
4	YA, RA	T23S rRNA	polymer	2915	948035.6	2	GenBank (55771382)	Thermus thermophilus HB8
5	YB, RB	5S rRNA	polymer	122	39494.5	2	GenBank (55771382)	Thermus thermophilus HB8
6	YD, RD	50S ribosomal protein L2	polymer	276	30532.6	2	UniProt (P60405) Pfam (PF00181) Pfam (PF03947) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
7	YE, RE	50S ribosomal protein L3	polymer	206	22450.2	2	UniProt (Q5SHN8) Pfam (PF00297) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
8	YF, RF	50S ribosomal protein L4	polymer	210	23271.9	2	UniProt (Q5SHN9) Pfam (PF00573) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)	L1e
9	YG, RG	50S ribosomal protein L5	polymer	182	21061.6	2	UniProt (Q5SHQ0) Pfam (PF00281) Pfam (PF00673) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
10	YH, RH	50S ribosomal protein L6	polymer	180	19568.9	2	UniProt (Q5SHQ3) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
11	YI, RI	50S ribosomal protein L9	polymer	148	16422.2	2	UniProt (Q5SLQ1) Pfam (PF01281) Pfam (PF03948) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
12	YN, RN	50S ribosomal protein L13	polymer	140	15927.9	2	UniProt (P60488) Pfam (PF00572) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
13	YO, RO	50S ribosomal protein L14	polymer	122	13323.6	2	UniProt (Q5SHP8) Pfam (PF00238) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
14	YP, RP	50S ribosomal protein L15	polymer	150	16319.1	2	UniProt (Q5SHQ7) Pfam (PF00828) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
15	YQ, RQ	50S ribosomal protein L16	polymer	141	15993.9	2	UniProt (P60489) Pfam (PF00252) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
16	YR, RR	50S ribosomal protein L17	polymer	118	13750.1	2	UniProt (Q9Z9H5) Pfam (PF01196) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
17	YS, RS	50S ribosomal protein L18	polymer	112	12639.8	2	UniProt (Q5SHQ4) Pfam (PF00861) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)	TL24
18	YT, RT	50S ribosomal protein L19	polymer	146	17188.8	2	UniProt (P60490) Pfam (PF01245) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
19	YU, RU	50S ribosomal protein L20	polymer	118	13779.3	2	UniProt (P60491) Pfam (PF00453) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
20	YV, RV	50S ribosomal protein L21	polymer	101	11069.2	2	UniProt (P60492) Pfam (PF00829) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
21	YW, RW	50S ribosomal protein L22	polymer	113	12808.1	2	UniProt (Q5SHP3) Pfam (PF00237) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
22	YX, RX	50S ribosomal protein L23	polymer	96	10759.8	2	UniProt (Q5SHP0) Pfam (PF00276) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
23	YY, RY	50S ribosomal protein L24	polymer	110	12085.6	2	UniProt (Q5SHP9) Pfam (PF00467) Pfam (PF17136) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
24	YZ, RZ	50S ribosomal protein L25	polymer	206	23238.6	2	UniProt (Q5SHZ1) Pfam (PF01386) Pfam (PF14693) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)	TL5
25	Y0, R0	50S ribosomal protein L27	polymer	85	9529.1	2	UniProt (P60493) Pfam (PF01016) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
26	Y1, R1	50S ribosomal protein L28	polymer	98	11004.2	2	UniProt (P60494) Pfam (PF00830) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
27	Y2, R2	50S ribosomal protein L29	polymer	72	8670.3	2	UniProt (Q5SHP6) Pfam (PF00831) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
28	Y3, R3	50S ribosomal protein L30	polymer	60	6799.1	2	UniProt (Q5SHQ6) Pfam (PF00327) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
29	Y4, R4	50S ribosomal protein L31	polymer	71	8300.5	2	UniProt (Q5SJE1) Pfam (PF01197) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
30	Y5, R5	50S ribosomal protein L32	polymer	60	6722.1	2	UniProt (P80339) Pfam (PF01783) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
31	Y6, R6	50S ribosomal protein L33	polymer	54	6632.9	2	UniProt (P35871) Pfam (PF00471) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
32	Y7, R7	50S ribosomal protein L34	polymer	49	6132.4	2	UniProt (P80340) Pfam (PF00468) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
33	Y8, R8	50S ribosomal protein L35	polymer	65	7506.2	2	UniProt (Q5SKU1) Pfam (PF01632) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
34	Y9, R9	50S ribosomal protein L36	polymer	37	4435.4	2	UniProt (Q5SHR2) Pfam (PF00444) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)	Ribosomal protein B
35	XA, QA	16S rRNA	polymer	1521	493864.6	2	GenBank (55771382)	Thermus thermophilus HB8
36	XB, QB	30S ribosomal protein S2	polymer	256	29317.7	2	UniProt (P80371) Pfam (PF00318) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
37	XC, QC	30S ribosomal protein S3	polymer	239	26751.1	2	UniProt (P80372) Pfam (PF07650) Pfam (PF00189) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
38	XD, QD	30S ribosomal protein S4	polymer	209	24373.4	2	UniProt (P80373) Pfam (PF00163) Pfam (PF01479) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
39	XE, QE	30S ribosomal protein S5	polymer	162	17583.4	2	UniProt (Q5SHQ5) Pfam (PF00333) Pfam (PF03719) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
40	XF, QF	30S ribosomal protein S6	polymer	101	11988.8	2	UniProt (Q5SLP8) Pfam (PF01250) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)	TS9
41	XG, QG	30S ribosomal protein S7	polymer	156	18051.0	2	UniProt (P17291) Pfam (PF00177) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
42	XH, QH	30S ribosomal protein S8	polymer	138	15868.6	2	UniProt (Q5SHQ2) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
43	XI, QI	30S ribosomal protein S9	polymer	128	14410.6	2	UniProt (P80374) Pfam (PF00380) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
44	XJ, QJ	30S ribosomal protein S10	polymer	105	11955.0	2	UniProt (Q5SHN7) Pfam (PF00338) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
45	XK, QK	30S ribosomal protein S11	polymer	129	13737.9	2	UniProt (P80376) Pfam (PF00411) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
46	XL, QL	30S ribosomal protein S12	polymer	132	14683.5	2	UniProt (Q5SHN3) Pfam (PF00164) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
47	XM, QM	30S ribosomal protein S13	polymer	126	14338.9	2	UniProt (P80377) Pfam (PF00416) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
48	XN, QN	30S ribosomal protein S14 type Z	polymer	61	7158.7	2	UniProt (Q5SHQ1) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
49	XO, QO	30S ribosomal protein S15	polymer	89	10578.4	2	UniProt (Q5SJ76) Pfam (PF00312) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
50	XP, QP	30S ribosomal protein S16	polymer	88	10410.0	2	UniProt (Q5SJH3) Pfam (PF00886) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
51	XQ, QQ	30S ribosomal protein S17	polymer	105	12325.7	2	UniProt (Q5SHP7) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
52	XR, QR	30S ribosomal protein S18	polymer	88	10258.3	2	UniProt (Q5SLQ0) Pfam (PF01084) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
53	XS, QS	30S ribosomal protein S19	polymer	93	10605.5	2	UniProt (Q5SHP2) Pfam (PF00203) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
54	XT, QT	30S ribosomal protein S20	polymer	106	11736.1	2	UniProt (P80380) Pfam (PF01649) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
55	XU, QU	30S ribosomal protein Thx	polymer	27	3350.0	2	UniProt (Q5SIH3) Pfam (PF17070) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)	S31
56	YA, QA, RA, QD, YB...	MAGNESIUM ION	non-polymer		24.3	2513	Chemie (MG)
57	XN, Y5, R5, Y6, R6...	ZINC ION	non-polymer		65.4	12	Chemie (ZN)
58	XD, QD	IRON/SULFUR CLUSTER	non-polymer		351.6	2	Chemie (SF4)

Sequence modifications

QY, XY: 1 - 365 (UniProt: P07012)

PDB	External Database	Details
Met -14	-	initiating methionine
Gly -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
Ser -4	-	expression tag
Glu -3	-	expression tag
Asp -2	-	expression tag
Pro -1	-	expression tag
Ala 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	110
	Total formula weight	4489581.6
Non-Polymers*	Number of molecules	2527
	Total formula weight	62566.7
All*	Total formula weight	4552148.3

*Water molecules are not included.