5DFE

70S termination complex containing E. coli RF2

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (QV, XV)	P-site tRNA fMet	polymer	77	24802.8	2	GenBank (1016582139)	Escherichia coli
2	B, E (QX, XX)	messenger RNA	polymer	25	8196.0	2		synthetic construct
3	C, F (QY, XY)	Peptide chain release factor 2	polymer	380	42991.4	2	UniProt (P07012) Pfam (PF03462) Pfam (PF00472)	Escherichia coli (strain K12)	RF-2
4	BC, G (YA, RA)	T23S rRNA	polymer	2915	948035.6	2	GenBank (55771382)	Thermus thermophilus HB8
5	CC, H (YB, RB)	5S rRNA	polymer	122	39494.5	2	GenBank (55771382)	Thermus thermophilus HB8
6	DC, I (YD, RD)	50S ribosomal protein L2	polymer	276	30532.6	2	UniProt (P60405) Pfam (PF00181) Pfam (PF03947)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
7	EC, J (YE, RE)	50S ribosomal protein L3	polymer	206	22450.2	2	UniProt (Q5SHN8) Pfam (PF00297)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
8	FC, K (YF, RF)	50S ribosomal protein L4	polymer	210	23271.9	2	UniProt (Q5SHN9) Pfam (PF00573)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)	L1e
9	GC, L (YG, RG)	50S ribosomal protein L5	polymer	182	21061.6	2	UniProt (Q5SHQ0) Pfam (PF00281) Pfam (PF00673)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
10	HC, M (YH, RH)	50S ribosomal protein L6	polymer	180	19568.9	2	UniProt (Q5SHQ3)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
11	IC, N (YI, RI)	50S ribosomal protein L9	polymer	148	16422.2	2	UniProt (Q5SLQ1) Pfam (PF01281) Pfam (PF03948)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
12	JC, O (YN, RN)	50S ribosomal protein L13	polymer	140	15927.9	2	UniProt (P60488) Pfam (PF00572)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
13	KC, P (YO, RO)	50S ribosomal protein L14	polymer	122	13323.6	2	UniProt (Q5SHP8) Pfam (PF00238)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
14	LC, Q (YP, RP)	50S ribosomal protein L15	polymer	150	16319.1	2	UniProt (Q5SHQ7) Pfam (PF00828)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
15	MC, R (YQ, RQ)	50S ribosomal protein L16	polymer	141	15993.9	2	UniProt (P60489) Pfam (PF00252)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
16	NC, S (YR, RR)	50S ribosomal protein L17	polymer	118	13750.1	2	UniProt (Q9Z9H5) Pfam (PF01196)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
17	OC, T (YS, RS)	50S ribosomal protein L18	polymer	112	12639.8	2	UniProt (Q5SHQ4) Pfam (PF00861)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)	TL24
18	PC, U (YT, RT)	50S ribosomal protein L19	polymer	146	17188.8	2	UniProt (P60490) Pfam (PF01245)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
19	QC, V (YU, RU)	50S ribosomal protein L20	polymer	118	13779.3	2	UniProt (P60491) Pfam (PF00453)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
20	RC, W (YV, RV)	50S ribosomal protein L21	polymer	101	11069.2	2	UniProt (P60492) Pfam (PF00829)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
21	SC, X (YW, RW)	50S ribosomal protein L22	polymer	113	12808.1	2	UniProt (Q5SHP3) Pfam (PF00237)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
22	TC, Y (YX, RX)	50S ribosomal protein L23	polymer	96	10759.8	2	UniProt (Q5SHP0) Pfam (PF00276)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
23	UC, Z (YY, RY)	50S ribosomal protein L24	polymer	110	12085.6	2	UniProt (Q5SHP9) Pfam (PF00467) Pfam (PF17136)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
24	AA, VC (YZ, RZ)	50S ribosomal protein L25	polymer	206	23238.6	2	UniProt (Q5SHZ1) Pfam (PF01386) Pfam (PF14693)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)	TL5
25	BA, WC (Y0, R0)	50S ribosomal protein L27	polymer	85	9529.1	2	UniProt (P60493) Pfam (PF01016)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
26	CA, XC (Y1, R1)	50S ribosomal protein L28	polymer	98	11004.2	2	UniProt (P60494) Pfam (PF00830)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
27	DA, YC (Y2, R2)	50S ribosomal protein L29	polymer	72	8670.3	2	UniProt (Q5SHP6) Pfam (PF00831)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
28	EA, ZC (Y3, R3)	50S ribosomal protein L30	polymer	60	6799.1	2	UniProt (Q5SHQ6) Pfam (PF00327)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
29	AD, FA (Y4, R4)	50S ribosomal protein L31	polymer	71	8300.5	2	UniProt (Q5SJE1) Pfam (PF01197)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
30	BD, GA (Y5, R5)	50S ribosomal protein L32	polymer	60	6722.1	2	UniProt (P80339) Pfam (PF01783)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
31	CD, HA (Y6, R6)	50S ribosomal protein L33	polymer	54	6632.9	2	UniProt (P35871) Pfam (PF00471)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
32	DD, IA (Y7, R7)	50S ribosomal protein L34	polymer	49	6132.4	2	UniProt (P80340) Pfam (PF00468)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
33	ED, JA (Y8, R8)	50S ribosomal protein L35	polymer	65	7506.2	2	UniProt (Q5SKU1) Pfam (PF01632)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
34	FD, KA (Y9, R9)	50S ribosomal protein L36	polymer	37	4435.4	2	UniProt (Q5SHR2) Pfam (PF00444)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)	Ribosomal protein B
35	GB, LA (XA, QA)	16S rRNA	polymer	1521	493864.6	2	GenBank (55771382)	Thermus thermophilus HB8
36	HB, MA (XB, QB)	30S ribosomal protein S2	polymer	256	29317.7	2	UniProt (P80371) Pfam (PF00318)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
37	IB, NA (XC, QC)	30S ribosomal protein S3	polymer	239	26751.1	2	UniProt (P80372) Pfam (PF07650) Pfam (PF00189)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
38	JB, OA (XD, QD)	30S ribosomal protein S4	polymer	209	24373.4	2	UniProt (P80373) Pfam (PF00163) Pfam (PF01479)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
39	KB, PA (XE, QE)	30S ribosomal protein S5	polymer	162	17583.4	2	UniProt (Q5SHQ5) Pfam (PF00333) Pfam (PF03719)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
40	LB, QA (XF, QF)	30S ribosomal protein S6	polymer	101	11988.8	2	UniProt (Q5SLP8) Pfam (PF01250)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)	TS9
41	MB, RA (XG, QG)	30S ribosomal protein S7	polymer	156	18051.0	2	UniProt (P17291) Pfam (PF00177)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
42	NB, SA (XH, QH)	30S ribosomal protein S8	polymer	138	15868.6	2	UniProt (Q5SHQ2)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
43	OB, TA (XI, QI)	30S ribosomal protein S9	polymer	128	14410.6	2	UniProt (P80374) Pfam (PF00380)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
44	PB, UA (XJ, QJ)	30S ribosomal protein S10	polymer	105	11955.0	2	UniProt (Q5SHN7) Pfam (PF00338)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
45	QB, VA (XK, QK)	30S ribosomal protein S11	polymer	129	13737.9	2	UniProt (P80376) Pfam (PF00411)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
46	RB, WA (XL, QL)	30S ribosomal protein S12	polymer	132	14683.5	2	UniProt (Q5SHN3) Pfam (PF00164)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
47	SB, XA (XM, QM)	30S ribosomal protein S13	polymer	126	14338.9	2	UniProt (P80377) Pfam (PF00416)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
48	TB, YA (XN, QN)	30S ribosomal protein S14 type Z	polymer	61	7158.7	2	UniProt (Q5SHQ1)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
49	UB, ZA (XO, QO)	30S ribosomal protein S15	polymer	89	10578.4	2	UniProt (Q5SJ76) Pfam (PF00312)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
50	AB, VB (XP, QP)	30S ribosomal protein S16	polymer	88	10410.0	2	UniProt (Q5SJH3) Pfam (PF00886)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
51	BB, WB (XQ, QQ)	30S ribosomal protein S17	polymer	105	12325.7	2	UniProt (Q5SHP7)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
52	CB, XB (XR, QR)	30S ribosomal protein S18	polymer	88	10258.3	2	UniProt (Q5SLQ0) Pfam (PF01084)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
53	DB, YB (XS, QS)	30S ribosomal protein S19	polymer	93	10605.5	2	UniProt (Q5SHP2) Pfam (PF00203)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
54	EB, ZB (XT, QT)	30S ribosomal protein S20	polymer	106	11736.1	2	UniProt (P80380) Pfam (PF01649)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
55	AC, FB (XU, QU)	30S ribosomal protein Thx	polymer	27	3350.0	2	UniProt (Q5SIH3) Pfam (PF17070)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)	S31
56	AAA, AAB, AAC, ABA, ABB... (YA, QA, RA, QD, YB...)	MAGNESIUM ION	non-polymer		24.3	2513	Chemie (MG)
57	ARA, BJA, BWC, CJA, EWC... (XN, Y5, R5, Y6, R6...)	ZINC ION	non-polymer		65.4	12	Chemie (ZN)
58	PQA, YBB (XD, QD)	IRON/SULFUR CLUSTER	non-polymer		351.6	2	Chemie (SF4)

Sequence modifications

QY, XY: 1 - 365 (UniProt: P07012)

PDB	External Database	Details
Met -14	-	initiating methionine
Gly -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
Ser -4	-	expression tag
Glu -3	-	expression tag
Asp -2	-	expression tag
Pro -1	-	expression tag
Ala 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	110
	Total formula weight	4489581.6
Non-Polymers*	Number of molecules	2527
	Total formula weight	62566.7
All*	Total formula weight	4552148.3

*Water molecules are not included.