5DFE

70S termination complex containing E. coli RF2

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-19
Detector	PSI PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	210.480, 450.410, 622.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.885 - 3.100
R-factor	0.197081129841
Rwork	0.196
R-free	0.22725
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y4o
RMSD bond length	0.005
RMSD bond angle	0.917
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.10.1_2155)
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.900		3.180
High resolution limit [Å]	3.100	13.860	3.100
Rmerge	0.269	0.061	1.002
Number of reflections	1057161
<I/σ(I)>	8.98	27.76	1.82
Completeness [%]	99.0	97.2	99.9
Redundancy	12.7
CC(1/2)	0.994	0.995	0.577

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	5.5% PEG 20k, 11% PEG 200, 100 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8 mM DOBC