5DFA

3D structure of the E323A catalytic mutant of Gan42B, a GH42 beta-galactosidase from G. stearothermophilus

Replaces:  4OJY

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Beta-galactosidase	polymer	686	80040.3	3	UniProt (F8TRX0) Pfam (PF02449) Pfam (PF08532) Pfam (PF08533)	Geobacillus stearothermophilus	Beta-gal
2	D, H, L (A, B, C)	ZINC ION	non-polymer		65.4	3	Chemie (ZN)
3	E, F, G, I, J... (A, B, C)	GLYCEROL	non-polymer		92.1	9	Chemie (GOL)
4	P, Q, R (A, B, C)	water	water		18.0	1120	Chemie (HOH)

Sequence modifications

A, B, C: 2 - 687 (UniProt: F8TRX0)

PDB	External Database	Details
Ala 323	Glu 322	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	240120.9
Non-Polymers*	Number of molecules	12
	Total formula weight	1025.1
All*	Total formula weight	241146.0

*Water molecules are not included.