5D6D

Crystal structure of GASDALIE IgG1 Fc in complex with FcgRIIIa

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Ig gamma-1 chain C region	polymer	249	28106.0	2	UniProt (P01857) Pfam (PF07654)	Homo sapiens (Human)
2	C (C)	Low affinity immunoglobulin gamma Fc region receptor III-A	polymer	240	27086.4	1	UniProt (P08637) Pfam (PF13895)	Homo sapiens (Human)	CD16a antigen,Fc-gamma RIII-alpha,FcRIIIa,FcR-10,IgG Fc receptor III-2
3	D (D)	beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[beta-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1625.5	1	In PDB GlyTouCan (G33244HE)
4	E (E)	beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1479.3	1	In PDB GlyTouCan (G89993FE)
5	F (B)	alpha-L-fucopyranose	non-polymer		164.2	1	Chemie (FUC)
6	G, H (C)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)

Sequence modifications

A, B: 225 - 446 (UniProt: P01857)

PDB	External Database	Details
Met 198	-	initiating methionine
Glu 199	-	expression tag
Phe 200	-	expression tag
Gly 201	-	expression tag
Leu 202	-	expression tag
Ser 203	-	expression tag
Trp 204	-	expression tag
Leu 205	-	expression tag
Phe 206	-	expression tag
Leu 207	-	expression tag
Val 208	-	expression tag
Ala 209	-	expression tag
Ile 210	-	expression tag
Leu 211	-	expression tag
Lys 212	-	expression tag
Gly 213	-	expression tag
Val 214	-	expression tag
Gln 215	-	expression tag
Cys 216	-	expression tag
Glu 217	-	expression tag
Val 218	-	expression tag
Gln 219	-	expression tag
Leu 220	-	expression tag
Leu 221	-	expression tag
Glu 222	-	expression tag
Thr 223	-	expression tag
Ser 224	-	expression tag
Ala 236	Gly 119	engineered mutation
Asp 239	Ser 122	engineered mutation
Leu 330	Ala 213	engineered mutation
Glu 332	Ile 215	engineered mutation

C: -4 - 187 (UniProt: P08637)

PDB	External Database	Details
Met -17	-	expression tag
Trp -16	-	expression tag
Gln -15	-	expression tag
Leu -14	-	expression tag
Leu -13	-	expression tag
Leu -12	-	expression tag
Pro -11	-	expression tag
Thr -10	-	expression tag
Ala -9	-	expression tag
Leu -8	-	expression tag
Leu -7	-	expression tag
Leu -6	-	expression tag
Leu -5	-	expression tag
Gln 38	Asn 56	engineered mutation
Gln 74	Asn 92	engineered mutation
Gln 169	Asn 187	engineered mutation
Ser 188	-	expression tag
Arg 189	-	expression tag
Gly 190	-	expression tag
Gly 191	-	expression tag
Gly 192	-	expression tag
Gly 193	-	expression tag
Ser 194	-	expression tag
Gly 195	-	expression tag
Gly 196	-	expression tag
Gly 197	-	expression tag
Gly 198	-	expression tag
His 199	-	expression tag
Val 200	-	expression tag
Leu 201	-	expression tag
Asn 202	-	expression tag
Asp 203	-	expression tag
Ile 204	-	expression tag
Phe 205	-	expression tag
Glu 206	-	expression tag
Ala 207	-	expression tag
Gln 208	-	expression tag
Lys 209	-	expression tag
Ile 210	-	expression tag
Glu 211	-	expression tag
Trp 212	-	expression tag
His 213	-	expression tag
Glu 214	-	expression tag
Thr 215	-	expression tag
Gly 216	-	expression tag
His 217	-	expression tag
His 218	-	expression tag
His 219	-	expression tag
His 220	-	expression tag
His 221	-	expression tag
His 222	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	83298.3
Branched	Number of molecules	2
	Total formula weight	3104.8
Non-Polymers*	Number of molecules	3
	Total formula weight	606.6
All*	Total formula weight	87009.7

*Water molecules are not included.