5D4E
Crystal structure of Thermus thermophilus product complex for transcription initiation with 3'-dephosphate-CoA and CTP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, I, J (A, B, K, L) | DNA-directed RNA polymerase subunit alpha | polymer | 315 | 35056.2 | 4 | UniProt (Q9Z9H6) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) UniProt (by SIFTS) (Q5SHR6) | Thermus thermophilus | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 2 | C, K (C, M) | DNA-directed RNA polymerase subunit beta | polymer | 1119 | 125436.5 | 2 | UniProt (Q8RQE9) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 3 | D, L (D, N) | DNA-directed RNA polymerase subunit beta' | polymer | 1524 | 170997.4 | 2 | UniProt (Q8RQE8) Pfam (PF04997) Pfam (PF21668) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 4 | E, M (E, O) | DNA-directed RNA polymerase subunit omega | polymer | 99 | 11533.3 | 2 | UniProt (Q8RQE7) Pfam (PF01192) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 5 | F, N (F, P) | RNA polymerase sigma factor SigA | polymer | 443 | 50769.4 | 2 | UniProt (Q72L95) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) UniProt (by SIFTS) (Q5SKW1) | Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) | |
| 6 | G, P (H, S) | DNA (27-MER) | polymer | 27 | 8421.4 | 2 | Thermus thermophilus | ||
| 7 | H, O (G, R) | DNA (5'-D(*CP*C*TP*GP*CP*AP*TP*CP*CP*GP*TP*GP*AP*GP*TP*AP*GP*AP*G)-3') | polymer | 19 | 5845.8 | 2 | Thermus thermophilus | ||
| 8 | AA, DA, GA, HA, Q... (L, N, P, B, D...) | MAGNESIUM ION | non-polymer | 24.3 | 9 | Chemie (MG) | |||
| 9 | BA, CA, R, S (N, D) | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) | |||
| 10 | EA, V (N, D) | CYTIDINE-5'-MONOPHOSPHATE | non-polymer | 323.2 | 2 | Chemie (C5P) | |||
| 11 | FA, W (N, D) | DIPHOSPHATE | non-polymer | 173.9 | 2 | Chemie (DPO) | |||
| 12 | IA, Z (R, G) | DEPHOSPHO COENZYME A | non-polymer | 687.6 | 2 | Chemie (COD) | |||
| 13 | JA, KA, LA, MA, NA... (A, B, C, D, E...) | water | water | 18.0 | 775 | Chemie (HOH) |
Sequence modifications
F, P: 1 - 423 (UniProt: Q72L95)
| PDB | External Database | Details |
|---|---|---|
| Met -19 | - | initiating methionine |
| Gly -18 | - | expression tag |
| Ser -17 | - | expression tag |
| Ser -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| Ser -9 | - | expression tag |
| Ser -8 | - | expression tag |
| Gly -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Val -5 | - | expression tag |
| Pro -4 | - | expression tag |
| Arg -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| His 0 | - | expression tag |
| Thr 46 | Ala 46 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 16 |
| Total formula weight | 886232.4 | |
| Non-Polymers* | Number of molecules | 19 |
| Total formula weight | 2849.8 | |
| All* | Total formula weight | 889082.1 |
