5D4B

Structural Basis for a New Templated Activity by Terminal Deoxynucleotidyl Transferase: Implications for V(D)J Recombination

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Terminal deoxynucleotidyltransferase	polymer	400	45704.0	2	UniProt (Q3UZ80) Pfam (PF14716) Pfam (PF10391) Pfam (PF01909) Pfam (PF14791) UniProt (by SIFTS) (P09838)	Mus musculus (Mouse)
2	C, D, E (C, D, E)	DNA (5'-D(*AP*AP*AP*AP*AP*C)-3')	polymer	6	1810.3	3		synthetic construct
3	F (F)	DNA (5'-D(*TP*TP*TP*TP*TP*GP*G)-3')	polymer	7	2134.4	1		synthetic construct
4	G, K (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	H, L (A, B)	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
6	I, J, M, N, O... (A, B, C)	SULFATE ION	non-polymer		96.1	8	Chemie (SO4)
7	S, T, U, V, W... (A, B, C, D, E...)	water	water		18.0	155	Chemie (HOH)

Sequence modifications

A, B: 132 - 510 (UniProt: Q3UZ80)

PDB	External Database	Details
Met 111	-	initiating methionine
Gly 112	-	expression tag
Ser 113	-	expression tag
Ser 114	-	expression tag
His 115	-	expression tag
His 116	-	expression tag
His 117	-	expression tag
His 118	-	expression tag
His 119	-	expression tag
His 120	-	expression tag
Ser 121	-	expression tag
Ser 122	-	expression tag
Gly 123	-	expression tag
Leu 124	-	expression tag
Val 125	-	expression tag
Pro 126	-	expression tag
Arg 127	-	expression tag
Gly 128	-	expression tag
Ser 129	-	expression tag
His 130	-	expression tag
Met 131	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	98973.2
Non-Polymers*	Number of molecules	12
	Total formula weight	863.1
All*	Total formula weight	99836.3

*Water molecules are not included.