5D16

Structure of the C-terminal domain of TnsE double mutant - A453V/D523N

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Transposon Tn7 transposition protein TnsE	polymer	206	23119.0	2	UniProt (P05845) Pfam (PF18623) In PDB	Escherichia coli	Protein D
2	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
3		water	water		18.0	202	Chemie (HOH)

Sequence modifications

A, B: 342 - 538 (UniProt: P05845)

PDB	External Database	Details
Met 341	-	initiating methionine
Val 453	Ala 453	engineered mutation
Asn 523	Asp 523	engineered mutation
Leu 539	-	expression tag
Glu 540	-	expression tag
His 541	-	expression tag
His 542	-	expression tag
His 543	-	expression tag
His 544	-	expression tag
His 545	-	expression tag
His 546	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	46238.0
Non-Polymers*	Number of molecules	1
	Total formula weight	92.1
All*	Total formula weight	46330.1

*Water molecules are not included.