5D16
Structure of the C-terminal domain of TnsE double mutant - A453V/D523N
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-06 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.030, 71.250, 50.430 |
Unit cell angles | 90.00, 97.28, 90.00 |
Refinement procedure
Resolution | 29.301 - 1.760 |
R-factor | 0.1988 |
Rwork | 0.198 |
R-free | 0.22000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5d17 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.757 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.490 | 1.820 |
High resolution limit [Å] | 1.760 | 1.760 |
Rmerge | 0.030 | 0.973 |
Number of reflections | 32735 | |
<I/σ(I)> | 21 | 1.4 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | PEG 3350, Bis-Tris |