5D0M

Structure of UbE2D2:RNF165:Ub complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Ubiquitin-conjugating enzyme E2 D2	polymer	152	17214.6	1	UniProt (P62837) Pfam (PF00179)	Homo sapiens (Human)	Ubiquitin carrier protein D2,Ubiquitin-conjugating enzyme E2(17)KB 2,Ubiquitin-conjugating enzyme E2-17 kDa 2,Ubiquitin-protein ligase D2,p53-regulated ubiquitin-conjugating enzyme 1
2	B (C)	RING finger protein 165	polymer	87	9799.1	1	UniProt (Q6ZSG1) Pfam (PF13639)	Homo sapiens (Human)
3	C (B)	Polyubiquitin-B	polymer	76	8576.8	1	UniProt (P0CG47) Pfam (PF00240)	Homo sapiens (Human)
4	D (A)	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
5	E, F (C)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
6	G, H, I (A, C, B)	water	water		18.0	100	Chemie (HOH)

Sequence modifications

A: 1 - 147 (UniProt: P62837)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Leu -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ser 21	Cys 21	engineered mutation
Arg 22	Ser 22	engineered mutation
Lys 85	Cys 85	engineered mutation
Ser 107	Cys 107	engineered mutation
Ser 111	Cys 111	engineered mutation

C: 255 - 346 (UniProt: Q6ZSG1)

PDB	External Database	Details
Gly 250	-	expression tag
Pro 251	-	expression tag
Leu 252	-	expression tag
Gly 253	-	expression tag
Ser 254	-	expression tag
-	Arg 273	deletion
-	Arg 274	deletion
-	Pro 275	deletion
-	Gln 276	deletion
-	Asp 277	deletion
-	Gly 278	deletion
-	Lys 279	deletion
-	Gly 280	deletion
Ala 313	Met 313	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	35590.6
Non-Polymers*	Number of molecules	3
	Total formula weight	225.8
All*	Total formula weight	35816.4

*Water molecules are not included.