5CWS

Crystal structure of the intact Chaetomium thermophilum Nsp1-Nup49-Nup57 channel nucleoporin heterotrimer bound to its Nic96 nuclear pore complex attachment site

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, G (A, G)	sAB-158 Fab Light Chain	polymer	237	25394.5	2		Homo sapiens
2	B, H (B, H)	sAB-158 Fab Heavy Chain	polymer	266	28606.1	2		Homo sapiens
3	C, I (C, I)	Nucleoporin NSP1	polymer	208	23558.2	2	UniProt (G0SBQ3) Pfam (PF05064)	Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)	Nuclear pore protein NSP1,Nucleoskeletal-like protein
4	D, J (D, J)	Nucleoporin NUP49	polymer	227	24536.5	2	UniProt (G0S4X2) Pfam (PF21121)	Chaetomium thermophilum	Nuclear pore protein NUP49
5	E, K (E, K)	Nucleoporin NUP57	polymer	247	28792.7	2	UniProt (G0S0R2) Pfam (PF13874) Pfam (PF18570)	Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)	Nuclear pore protein NUP57
6	F, L (F, L)	Nucleoporin NIC96	polymer	74	7793.8	2	UniProt (G0S024)	Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)	Nuclear pore protein NIC96
7	M, N (C, I)	OSMIUM ION	non-polymer		190.2	2	Chemie (OS)

Sequence modifications

D, J: 246 - 472 (UniProt: G0S4X2)

PDB	External Database	Details
Met 244	-	initiating methionine
Ser 245	-	expression tag

E, K: 71 - 316 (UniProt: G0S0R2)

PDB	External Database	Details
Met 70	-	initiating methionine

F, L: 141 - 213 (UniProt: G0S024)

PDB	External Database	Details
Ser 140	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	277363.5
Non-Polymers*	Number of molecules	2
	Total formula weight	380.5
All*	Total formula weight	277744.0

*Water molecules are not included.