5CWS
Crystal structure of the intact Chaetomium thermophilum Nsp1-Nup49-Nup57 channel nucleoporin heterotrimer bound to its Nic96 nuclear pore complex attachment site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-03-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.1400 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 123.180, 162.820, 212.870 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.666 - 3.770 |
R-factor | 0.2309 |
Rwork | 0.229 |
R-free | 0.26540 |
Structure solution method | AB INITIO PHASING |
RMSD bond length | 0.003 |
RMSD bond angle | 0.678 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 3.770 | 3.770 |
Rmerge | 0.204 | |
Number of reflections | 43896 | |
<I/σ(I)> | 17 | |
Completeness [%] | 99.9 | |
Redundancy | 40.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.7 | 294 | 0.1M TRIS, pH 8.7 5.7 % (w/v) PEG 20,000 1 % (v/v) 1-propanol |