5CUR

G158E/K44E/R57E/Y49E Bacillus subtilis lipase A with 20% [BMIM][Cl]

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Esterase	polymer	180	19321.6	1	UniProt (I6V559) Pfam (PF01674) UniProt (by SIFTS) (P37957) In PDB	Bacillus subtilis	Lipase,Lipase A
2	A	1-butyl-3-methyl-1H-imidazol-3-ium	non-polymer		139.2	5	Chemie (BM0)
3	A	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
4		water	water		18.0	234	Chemie (HOH)

Sequence modifications

A: 2 - 181 (UniProt: I6V559)

PDB	External Database	Details
Glu 44	Lys 75	engineered mutation
Glu 49	Tyr 80	engineered mutation
Glu 57	Arg 88	engineered mutation
Glu 158	Gly 189	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	19321.6
Non-Polymers*	Number of molecules	7
	Total formula weight	767.0
All*	Total formula weight	20088.6

*Water molecules are not included.