5CUR
G158E/K44E/R57E/Y49E Bacillus subtilis lipase A with 20% [BMIM][Cl]
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004806 | molecular_function | triacylglycerol lipase activity |
| A | 0005576 | cellular_component | extracellular region |
| A | 0006629 | biological_process | lipid metabolic process |
| A | 0016042 | biological_process | lipid catabolic process |
| A | 0016298 | molecular_function | lipase activity |
| A | 0016787 | molecular_function | hydrolase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | binding site for residue BM0 A 201 |
| Chain | Residue |
| A | TYR37 |
| A | PHE58 |
| A | LYS61 |
| A | GLU65 |
| A | GLN121 |
| A | HOH392 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | binding site for residue BM0 A 202 |
| Chain | Residue |
| A | BM0203 |
| A | HOH419 |
| A | HOH532 |
| A | ALA20 |
| A | GLY21 |
| A | TYR161 |
| site_id | AC3 |
| Number of Residues | 3 |
| Details | binding site for residue BM0 A 203 |
| Chain | Residue |
| A | PHE17 |
| A | TYR139 |
| A | BM0202 |
| site_id | AC4 |
| Number of Residues | 11 |
| Details | binding site for residue BM0 A 204 |
| Chain | Residue |
| A | ILE12 |
| A | GLY13 |
| A | ASN18 |
| A | HIS76 |
| A | SER77 |
| A | HIS156 |
| A | ILE157 |
| A | LEU160 |
| A | CL207 |
| A | HOH388 |
| A | HOH516 |
| site_id | AC5 |
| Number of Residues | 7 |
| Details | binding site for residue BM0 A 205 |
| Chain | Residue |
| A | HIS3 |
| A | GLN29 |
| A | GLY30 |
| A | TRP31 |
| A | LEU173 |
| A | ASN174 |
| A | HOH311 |
| site_id | AC6 |
| Number of Residues | 2 |
| Details | binding site for residue CL A 206 |
| Chain | Residue |
| A | MET134 |
| A | HIS156 |
| site_id | AC7 |
| Number of Residues | 5 |
| Details | binding site for residue CL A 207 |
| Chain | Residue |
| A | HIS10 |
| A | GLY11 |
| A | ASN18 |
| A | HIS76 |
| A | BM0204 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | Active site: {"description":"Nucleophile","evidences":[{"source":"PubMed","id":"11491291","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"11583117","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"12077437","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | Active site: {"description":"Charge relay system","evidences":[{"source":"PubMed","id":"11491291","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"11583117","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"12077437","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 5 |
| Details | M-CSA 518 |
| Chain | Residue | Details |
| A | ILE12 | electrostatic stabiliser |
| A | SER77 | covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor |
| A | MET78 | electrostatic stabiliser |
| A | ASP133 | electrostatic stabiliser, increase basicity, modifies pKa |
| A | HIS156 | proton acceptor, proton donor |
