5CTV

Catalytic domain of LytA, the major autolysin of Streptococcus pneumoniae, (C60A, H133A, C136A mutant) complexed with peptidoglycan fragment

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Autolysin	polymer	188	21199.3	1	UniProt (P06653)	Streptococcus pneumoniae serotype 4	Cell wall hydrolase,Mucopeptide aminohydrolase,Murein hydrolase,N-acetylmuramoyl-L-alanine amidase
2	B, C (C, E)	fragment of peptidoglycan	polymer	5	489.5	2		Streptococcus pneumoniae
3	D (B)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-methyl 2-acetamido-3-O-[(1R)-1-carboxyethyl]-2-deoxy-beta-D-glucopyranoside-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-methyl 2-acetamido-3-O-[(1R)-1-carboxyethyl]-2-deoxy-beta-D-glucopyranoside	branched		1003.0	1
4	E, F, G (A, C, E)	water	water		18.0	248	Chemie (HOH)

Sequence modifications

A: 1 - 180 (UniProt: P06653)

PDB	External Database	Details
Ala -1	-	expression tag
Ser 0	-	expression tag
Ala 60	Cys 60	engineered mutation
Ala 133	His 133	engineered mutation
Ala 136	Cys 136	engineered mutation
His 181	-	expression tag
His 182	-	expression tag
His 183	-	expression tag
His 184	-	expression tag
His 185	-	expression tag
His 186	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	22178.3
Branched	Number of molecules	1
	Total formula weight	1003.0
All*	Total formula weight	23181.3

*Water molecules are not included.