5CSE

Streptavidin-S112Y-K121E Complexed with Palladium-Containing Biotin Ligand

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Streptavidin	polymer	159	16646.0	2	UniProt (P22629) Pfam (PF01382) In PDB	Streptomyces avidinii
2	A, B	chloro{di-tert-butyl[2-({5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoyl}amino)ethyl]-lambda~5~-phosphanyl}(1-phenylprop-1-ene-1,3-diyl-kappa~2~C~1~,C~3~)palladium	non-polymer		673.6	2	Chemie (SVP)
3	A, B	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
4		water	water		18.0	50	Chemie (HOH)

Sequence modifications

A, B: 14 - 134 (UniProt: P22629)

PDB	External Database	Details
Met 1	-	initiating methionine
Ala 2	-	expression tag
Ser 3	-	expression tag
Met 4	-	expression tag
Thr 5	-	expression tag
Gly 6	-	expression tag
Gly 7	-	expression tag
Gln 8	-	expression tag
Gln 9	-	expression tag
Met 10	-	expression tag
Gly 11	-	expression tag
Arg 12	-	expression tag
Asp 13	-	expression tag
Tyr 112	Ser 136	engineered mutation
Glu 121	Lys 145	engineered mutation
Pro 135	-	expression tag
Ser 136	-	expression tag
Ala 137	-	expression tag
Ala 138	-	expression tag
Ser 139	-	expression tag
Ile 140	-	expression tag
Asp 141	-	expression tag
Ala 142	-	expression tag
Ala 143	-	expression tag
Lys 144	-	expression tag
Lys 145	-	expression tag
Ala 146	-	expression tag
Gly 147	-	expression tag
Val 148	-	expression tag
Asn 149	-	expression tag
Asn 150	-	expression tag
Gly 151	-	expression tag
Asn 152	-	expression tag
Pro 153	-	expression tag
Leu 154	-	expression tag
Asp 155	-	expression tag
Ala 156	-	expression tag
Val 157	-	expression tag
Gln 158	-	expression tag
Gln 159	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	33292.1
Non-Polymers*	Number of molecules	4
	Total formula weight	1418.1
All*	Total formula weight	34710.2

*Water molecules are not included.