5CO4

Structural insights into the 2-OH methylation of C/U34 on tRNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (B, A)	Putative tRNA (cytidine(34)-2'-O)-methyltransferase	polymer	178	20055.2	2	UniProt (Q72G96) Pfam (PF00588)	Thermus thermophilus HB27	TrmL, tRNA (cytidine/uridine-2'-O-)-methyltransferase
2	C, E (B, A)	5'-DEOXY-5'-METHYLTHIOADENOSINE	non-polymer		297.3	2	Chemie (MTA)
3	D (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4	F, G (B, A)	water	water		18.0	314	Chemie (HOH)

Sequence modifications

B, A: 1 - 149 (UniProt: Q72G96)

PDB	External Database	Details
Phe -28	-	expression tag
Cys -27	-	expression tag
Leu -26	-	expression tag
Thr -25	-	expression tag
Leu -24	-	expression tag
Arg -23	-	expression tag
Arg -22	-	expression tag
Arg -21	-	expression tag
Tyr -20	-	expression tag
Thr -19	-	expression tag
Met -18	-	expression tag
Gly -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Leu -8	-	expression tag
Glu -7	-	expression tag
Val -6	-	expression tag
Leu -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Pro -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	40110.4
Non-Polymers*	Number of molecules	3
	Total formula weight	686.8
All*	Total formula weight	40797.2

*Water molecules are not included.