5CDP
2.45A structure of etoposide with S.aureus DNA gyrase and DNA
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, C | DNA gyrase subunit A | polymer | 483 | 54738.5 | 2 | UniProt (Q99XG5) Pfam (PF00521) In PDB | Staphylococcus aureus (strain N315) | |
2 | B, D | DNA gyrase subunit B | polymer | 190 | 21345.3 | 2 | UniProt (P66937) Pfam (PF01751) Pfam (PF00986) In PDB | Staphylococcus aureus (strain N315) | Greek key deletion construct |
3 | E, F | DNA (5'-D(*AP*GP*CP*CP*GP*TP*AP*G*GP*GP*TP*AP*CP*CP*TP*AP*CP*GP*GP*CP*T)-3') | polymer | 8 | 2451.6 | 2 | synthetic construct | ||
4 | G, H | DNA (5'-D(*AP*GP*CP*CP*GP*TP*AP*G*GP*GP*TP*AP*CP*CP*TP*AP*CP*GP*GP*CP*T)-3') | polymer | 13 | 3967.6 | 2 | synthetic construct | ||
5 | A, H | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) | |||
6 | B, D, G | MANGANESE (II) ION | non-polymer | 54.9 | 3 | Chemie (MN) | |||
7 | H | (5S,5aR,8aR,9R)-9-(4-hydroxy-3,5-dimethoxyphenyl)-8-oxo-5,5a,6,8,8a,9-hexahydrofuro[3',4':6,7]naphtho[2,3-d][1,3]dioxol -5-yl 4,6-O-[(1R)-ethylidene]-beta-D-glucopyranoside | non-polymer | 588.6 | 1 | Chemie (EVP) | |||
8 | water | water | 18.0 | 767 | Chemie (HOH) |
Sequence modifications
A, C: 9 - 491 (UniProt: Q99XG5)
B, D: 417 - 543 (UniProt: P66937)
B, D: 580 - 640 (UniProt: P66937)
PDB | External Database | Details |
---|---|---|
Phe 123 | Tyr 123 | engineered mutation |
B, D: 580 - 640 (UniProt: P66937)
PDB | External Database | Details |
---|---|---|
Thr 544 | - | linker |
Gly 545 | - | linker |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 8 |
Total formula weight | 165005.9 | |
Non-Polymers* | Number of molecules | 6 |
Total formula weight | 799.4 | |
All* | Total formula weight | 165805.2 |