5CA3
Crystal structure of the glycosynthase mutant D324N of Escherichia coli GH63 glycosidase in complex with glucose and lactose
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Glucosidase YgjK | polymer | 760 | 86003.8 | 2 | UniProt (P42592) Pfam (PF21152) Pfam (PF22422) In PDB | Escherichia coli (strain K12) | |
2 | C, D | beta-D-galactopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 2 | In PDB BIRD (PRD_900008) GlyTouCan (G88362QR) | alpha-lactose | ||
3 | A, B | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
4 | A, B | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
5 | B | beta-D-glucopyranose | non-polymer | 180.2 | 1 | Chemie (BGC) | |||
6 | B | alpha-D-glucopyranose | non-polymer | 180.2 | 1 | Chemie (GLC) | |||
7 | water | water | 18.0 | 1286 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 760 (UniProt: P42592)
PDB | External Database | Details |
---|---|---|
Asn 324 | Asp 347 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 172007.7 | |
Branched | Number of molecules | 2 |
Total formula weight | 684.6 | |
Non-Polymers* | Number of molecules | 7 |
Total formula weight | 513.4 | |
All* | Total formula weight | 173205.6 |