5CA3
Crystal structure of the glycosynthase mutant D324N of Escherichia coli GH63 glycosidase in complex with glucose and lactose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Glucosidase YgjK | polymer | 760 | 86003.8 | 2 | UniProt (P42592) Pfam (PF21152) Pfam (PF22422) | Escherichia coli (strain K12) | |
| 2 | C, D (C, D) | beta-D-galactopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 2 | In PDB BIRD (PRD_900008) GlyTouCan (G88362QR) | alpha-lactose | ||
| 3 | E, H (A, B) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 4 | F, G, I (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 5 | J (B) | beta-D-glucopyranose | non-polymer | 180.2 | 1 | Chemie (BGC) | |||
| 6 | K (B) | alpha-D-glucopyranose | non-polymer | 180.2 | 1 | Chemie (GLC) | |||
| 7 | L, M (A, B) | water | water | 18.0 | 1286 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 760 (UniProt: P42592)
| PDB | External Database | Details |
|---|---|---|
| Asn 324 | Asp 347 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 172007.7 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 684.6 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 513.4 | |
| All* | Total formula weight | 173205.6 |
