5C8X

ZHD-Intermediate complex after ZHD crystal soaking in ZEN for 20min

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Zearalenone hydrolase	polymer	284	31019.0	2	UniProt (Q8NKB0) Pfam (PF00561)	Clonostachys rosea
2	C, F (A, B)	(1E,10S)-1-(3,5-dihydroxyphenyl)-10-hydroxyundec-1-en-6-one	non-polymer		292.4	2	Chemie (ZFR)
3	D, G (A, B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
4	E, H (A, B)	POTASSIUM ION	non-polymer		39.1	2	Chemie (K)
5	I, J (A, B)	water	water		18.0	336	Chemie (HOH)

Sequence modifications

A, B: 1 - 264 (UniProt: Q8NKB0)

PDB	External Database	Details
Ile 26	Val 26	See Sequence details
Ala 69	Pro 69	See Sequence details
Ile 87	Val 87	See Sequence details
Asn 148	Lys 148	See Sequence details
Leu 168	Met 168	See Sequence details
Val 170	Asp 170	See Sequence details
Gln 198	Lys 198	See Sequence details
Val 200	Leu 200	See Sequence details
Trp 265	-	expression tag
Asn 266	-	expression tag
Ser 267	-	expression tag
Ser 268	-	expression tag
Ser 269	-	expression tag
Val 270	-	expression tag
Asp 271	-	expression tag
Lys 272	-	expression tag
Leu 273	-	expression tag
Ala 274	-	expression tag
Ala 275	-	expression tag
Ala 276	-	expression tag
Leu 277	-	expression tag
Glu 278	-	expression tag
His 279	-	expression tag
His 280	-	expression tag
His 281	-	expression tag
His 282	-	expression tag
His 283	-	expression tag
His 284	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	62037.9
Non-Polymers*	Number of molecules	6
	Total formula weight	847.1
All*	Total formula weight	62885.1

*Water molecules are not included.